Biography:

Will be updated soon

Abstract:

Liquid crystals (LC) are a special unique mesophase that combines both the properties of a liquid (viscosity and fluidity) and a solid (anisotropy of the refractive index, diffraction, etc.). From one side, the molecules of liquid crystals with the dimension of ~15 angstroms can be considered as the real nano-objects; from the other side, they can be ordered by the action of the different external fields, including electric field, which allows creating the various electro-optical devices. Electro- and light-addressed spatial light modulators, display elements, biomedical indicators, sensors, etc. can be developed based on the LC mesophase. It should be mentioned that each LC cell includes a direct LC layer, conductive contacts based on indium tin oxides (ITO) or zinc oxide (ZnO), orientation layers, glass or quartz substrates. ITO contacts with the thickness significantly less than the thickness of the LC layer are often destroyed under the influence of intense laser radiation. Changes in the strength parameters of ITO are a problem that can be solved, for example, by various technological approaches, such as: HfO₂ deposition using PDV or CVD methods. Among technical methods, the laser-oriented deposition (LOD) technique occupies a special place. This method permits to deposit the carbon nanotubes (CNTs) directly in the vertical position on the substrate without essential losses of the carbon materials. The advantage of this method is connected with the fact that modified ITO-conducting layers can predict the novel properties.

In the current report based on this novel nanotechnological approach, it is possible to increase the mechanical and laser strength of ITO contacts, dramatically decrease the resistance, and reduce the number of technological operations when developing an LCD device. In addition, it is important to note that ITO contacts are capable of performing two functions: an electric conductor and an orientating layer for the LC molecules. Thus, it is possible to remove a direct orientating high-resistance layer (usually polyimide) from the technological process, reducing the level of the supply voltage applied to the device.

In this paper, a comparative analysis of the physical and technical parameters of pure ITO, ITO structured by hafnium oxides, ITO structured by vertically deposited CNTs is carried out. Preliminarily performed experiments, analytical, and quantum-chemical calculations allow us to expand the field of use of LC cells with modified ITO layers not only for the general purposes of optoelectronics but also for the optical limitation of high-power laser radiation and for the orientation of bio-objects based on DNA and red blood cells.

Biography:

Bo Yan, 2000-2004 graduated from Zhejiang University, 2004-2009 Chinese Academy of Sciences, Shanghai Institute of Optics and Fine Mechanics, 2009-2011, University of Science and Technology, postdoctoral, 2011-2015, JILA, University of Colorado, postdoctoral, 2015, national "1000-young " plan, back to Zhejiang University to set up a new lab.

Professor Bo Yan’s research is mainly focused on an experimental study of the ultra-cold atoms, cold molecules, including strongly correlated ultra-cold atomic systems and laser cooling of polar molecules. Prof. Bo Yan has published more than 20 papers, including Nature, Science, Phys. Rev. Lett, the total citation is more than 1300.

Abstract:

By introducing the Raman couplings, Bose-Einstein condensate can be coupled into different momentum states. A lattice in the momentum space can be created by introducing multi-frequency Raman couplings. By engineering the tunneling rates, the detunings, and the relative phase of the couplings in different sites, topological models can be realized and studied experimentally. By switching the couplings on and off, we realize a topological quantum walk of ultracold atoms in the momentum space.

The topological phase transition is mapped out, and the interaction induced localization effect is observed. By introducing the effective loss, a non-Hermitian AB ring is realized, and the non-reciprocal transport behavior is observed.

Biography:

Francesco Dell’ Olio received the M.Sc. degree in electronic engineering (cumlaude) and the Ph.D. degree in information engineering from Polytechnic University of Bari, Bari, Italy, in 2005 and 2010, respectively. Since December 2019, he has been an Assistant Professor at the Polytechnic University of Bari. His research interests include integrated optoelectronics and photonics. He has been involved in several research projects and is the co-author of more than 100 journal articles and conference papers.

Abstract:

In the last two decades, there has been a game-changing transformation of integrated microphotonics. Silicon has become the material of choice to fabricate photonic integrated circuits (PICs) whose complexity is increasing according to Moore’s law.

Silicon photonics components and integrated circuits are typically designed, assuming that the propagating mode is either TE or TM. This is due to the high birefringence of the silicon wires commonly used to guide light within them.

Polarization handling devices improve the performance of the silicon PICs by reducing the polarization-dependent dispersion and loss. In addition, the research interest towards polarization division multiplexed transceivers using PICs in stimulating a quickly growing effort on the technical solutions enabling polarization management on-chip.

The talk overviews the approaches currently utilized for designing efficient polarization handling devices in high-density silicon PICs and discuss the most promising strategies for improving the performance of such devices. A few notes on a new ultralow loss and high extinction ratio silicon TM-pass polarizer are given.

Biography:

Matras Guillaume joined Thales Laser (Orsay, France) in 2004 as a Ph.D. student to develop a high repetition rate Ti:Sa laser system for micro-processing applications in collaboration with Hubert Curien Laboratory (Saint-Etienne, France). He received his Ph.D. in 2008 and continued in the same company to work on several and complex Ti:Sa laser systems (mJ-kHz, TW, and PW systems) for scientific customers, firstly as laser engineers and progressively as the project manager. In 2013, he was called to manage the development and the delivery of the stretchers and compressors of the High Power Laser System for ELI-NP. In 2016, he was appointed as a technical solution leader to ensure the good technical implementation of the laser system in the ELI-NP facility. The HPLS was delivered in October 2019 with full performances. In parallel (2017- 2018), he had the opportunity to work with the ICFO laboratory of Barcelona to develop a mid-IR OPCPA source at 7µm.

Abstract:

We report the generation of unprecedented 10 Peta Watt laser pulses obtained from each of the 2 beamlines of the High Power Laser System (HPLS) of ELI-NP (Extreme Light Infrastructure – Nuclear Physics) research infrastructure. The laser system is a hybrid system made of a double CPA based on amplification within Titanium Sapphire crystals combined with an OPCPA with a parametric amplification stage boosting the energy to 10 mJ at the entrance of the second CPA. A XPW filter is also inserted between the 2 CPA and, in combination with the OPCPA, improves the temporal contrast of the pulses by typically 7 orders of magnitude. The spectral effects occurring during amplification, such as gain narrowing and wavelength shifting, are compensated through the use of spectral filters. Final amplification stages are involving large aperture TiSa crystals (up to 200 mm), which are pumped by high energy frequency-doubled Nd: Glass lasers delivering each 100 J of green light. Laser beams have been amplified respectively up to 332 J and to 342 J of pulse energy at 1 shot per minute without any occurrence of ASE and transverse lasing thanks to index matching fluid surrounding the crystal over is the entire length and pump deposition split over the time before each beam pass within the TiSa crystal. We have demonstrated full aperture compression by metric gratings of these amplified pulses down to 22.3 fs and therefore made the full demonstration for the first time ever of 10 PW capabilities from a laser system.

Biography:

Jingbo Qijoints University of Electronic Science and Technology of China (UESTC) as a Professor in the School of Electronic Science and Engineering. Prior to attending STC, he was a Professor at the PEAC Institute of Multiscale Sciences. Before that, he was a postdoctoral research associate in the Los Alamos National Laboratory and National High Magnetic Field Laboratory, respectively. He received his Ph. D. from Vanderbilt University. His primary research interests are in the field of ultrafast spectroscopy and THz spectroscopy of the condensed matter systems. Specifically, he is interested in the coherent control of quantum materials and ultrafast manipulation of light (electromagnetic wave) properties via quantum materials.

Abstract:

As a fascinating topological phase of matter, Weyl semimetals host chiral fermions with distinct chiralities and spin textures. Optical excitations involving those chiral fermions can induce exotic carrier responses, and in turn, lead to novel optical phenomena. Here, we discover strong coherent terahertz emission from Weyl semimetal TaAs, which is demonstrated as a unique broadband source of the chiral terahertz wave. The polarization control of the THz emission is achieved by tuning photoexcitation of ultrafast photocurrents via the photogalvanic effect. In the near-infrared regime, the photon-energy dependent non-thermal current due to the predominant circular photogalvanic effect can be attributed to the radical change of the band velocities when the chiral Weyl fermions are excited during selective optical transitions between the tilted anisotropic Weyl cones and the massive bulk bands. Our findings provide a design concept for creating chiral photon sources using quantum materials and open up new opportunities for developing ultrafast optoelectronics using Weyl physics.

Biography:

Xiaodong Zhang, a Professor, has completed his M.E. and a Ph.D. degree in Mechanics from Xi’an Jiaotong University, Xi’an, China. He has published more than 100 papers, includes 30 papers indexed by SCI & about 50 papers indexed by EI, and won 20 Patents of invention & 10 Design Patents. And He serves as an Associate Editor of the Journal of Mechanical Science and Technology & Editor of Intelligent Service Robotics.

Abstract:

In recent years, with the increasing popularity of wind power technology and the rapid development of wind power industry, the production of Direct-Driven Wind Turbines (DDWT)is increasing year by year in the world. The air gap between the stator and rotor is an important parameter to characterize its fault information, whose size generally ranges from 4 to 8 mm. In this paper, the measurement range of the current double-loop coaxial optic fiber displacement sensor is firstly analyzed, so that an approach for increasing the measurement range of the optic fiber sensor is proposed by increasing the number of receiving optic fiber circles. And then, the mathematical model of output modulation function is deduced when the sensor has a different number of receiving optical fibers, and the simulation results for the modulation function of the multi-loop receiving optical fibers sensor show that the measurement range of the dual-loop coaxial, optical fibers displacement sensor can be increased with 71.9% by comparing with the current one. Finally, in the calibration experiment of the sensor, the results of the actual measurement range are consistent with the theoretical simulation.

Biography:

Paulo P. Monteiro received the diploma “Licenciatura” in Electronics and Telecommunications Engineering from the University of Aveiro in 1988, an M.Sc. in Electronic Engineering, from the University of Wales UK, in 1990 and the Ph.D. in Electrical Engineering, from the University of Aveiro, in 1999. Presently, he is an Associate Professor at the University of Aveiro and Senior Researcher at the Instituto de Telecomunicações. His main research interests include Optical Communication Networks and Microwave Photonics. He tutored and co-tutored successfully more than 14 Ph.D.’s, having participated in more than 28 research projects (national and international). He has authored/co-authored more than 18 patent applications and over 110 papers in journals and 380 conference contributions. He is a member of the ECOC Technical Program Committee and Senior Member of IEEE.

Abstract:

Photonics is expected to revolutionize approaches for next-generation Radar and LiDAR systems since it can provide high spectral purity on signal generation and signal integrity on the detection and processing of high-frequency signals.

Photonics is also enabling the implementation of a distributed network of coherent multi static MIMO (Multiple Input-Multiple Output) radars with co-located or widely separated antennas exploiting spatially distributed information enhancing the detection capability in case of complex targets.

This talk discusses the main trends of using Photonics in Radar and LiDAR Systems and the approach followed in the projects RETIOT (Reflectometry Technologies to Enhance the Future Internet of Things and Cyber-Physical Systems) and LANDmaRk (Light communic AtioN Detection and Ranging).

Biography:

Will be updated soon 

Abstract:

To obtain nanostructures and thin films on a solid surface with controlled topology, we used several techniques. Namely, in addition to direct laser modification of solid target surfaces, the schemes with laser ablation of various targets (noble metals and their complexes with graphite and silicon, bimetals, refractory metals, semiconductors, graphite, etc.) in various liquids (from water to ethanol and glycerin, liquid nitrogen) with widely ranging viscosity were also implemented. In this case, first, a colloidal system was obtained; then, nanoparticles were deposited from the colloid on a solid surface to form the nanostructures of required topology in two ways: by laser radiation and by a drop method from the colloid through an appropriate nozzle. These processes in colloid systems with metal nanoparticles during laser ablation are associated with variations in local heating, an increase in the exposure time of each specific local volume, and control of laser-induced thermal diffusion of nanoparticles in the liquid. In general, for the case, both non-steady states random and non-ergodic processes in dynamic cluster systems under conditions of size quantization take place and result in new phase transitions. Moreover, laser-induced nanostructures and thin films with controllable topology vs. time may be presented as 4D-laser technology fabrication of new structures and materials. This is due to the reason that, e.g. thermodiffusion, gas-dynamic evaporation in pore-like structures with bubbles, ablation products, ballistic movement of the particles in liquid depend on the laser pulses duration. Thus, the interaction effects of solid targets with laser pulses of different duration for obtaining of various nanocluster structures can be viewed as the possibility of synthesizing the 4D-objects, when the result depends not only on the stationary topological/geometric parameters of the system but also on the dynamic interactions in the system leading to different final stable structures.

2. Using numerical methods, we have demonstrated the procedures for obtaining thin granular metal films with an arbitrary topology upon variation in the control parameters of the problem, as well as the possibility of predicting both their optical properties and electrical conductivity by computer simulation. The electrical conductivity and reflection, transmission, and absorption coefficients in the visible spectral range were calculated for various cases. For this, modern computer technologies were used in combination with original computer simulation algorithms. As a result, a good agreement with experimental data was obtained.

3. In the experiment with nanostructures in thin films on the surface of a substrate, we observed competition between two processes: first, an increase in electrical conductivity as a result of the opening of new channels in a spatially inhomogeneous conducting system and, second, an increase in resistivity due to an increase in the distance between conducting islands. Such electrical transport properties are determined by correlated quantum states leading to the tunnel and hopping electrical conductivities. They can be represented as a special type of topological electrophysical surface structures (both localized and delocalized) for the bound states of charge carriers. A sharp increase in electrical conductivity (by several orders of magnitude) was detected in our experiments due to a change in the topological features of the thin-film nanocluster system. We consider some physical principles for such processes in partially non-uniform complexes with different elemental compositions of thin multilayer cluster films on a solid surface when free charged particles propagate along the boundaries of conducting surfaces. A fundamentally new point of our study is the finding of trends in superconductivity based on fundamental effects in nanocluster structures with a specifically selected topology.

4. Granular thin films formed from spherical metal nanoparticles (e.g., of noble metals) deposited on both the dielectric substrate and the surface of individual other nanoobjects of significantly larger size, e.g., in the Au + Si combination, are of special significance for the optical characteristics. These structures with a controlled optical response can be used in the development of various sensors, electrochromic devices, supercapacitors, photoelectrochemical converters of solar energy, etc.

5. The results of this work make it possible to consider new physical principles for creating topological optoelectronic and photonic functional elements in a hybrid (optics + electrophysics) circuit using various spatial structures of an ensemble of nanoclusters in thin films on a solid substrate at room temperature, which, in fact, are incommensurable structures with laser-controlled symmetry. The aforementioned features of the fundamental effects in nanostructured thin-film solid-state systems make it possible to speak about the formation of a new line of research, i.e. topological photonics, which has undoubted applied promise, in particular, for femtosecond nanoelectronics.

Biography:

Prof. Yosef Ben Ezra, Dean of Engineering Faculty at Holon Institute of Technology, received his Ph.D. from Tel-Aviv University. During 2003-2005 Prof. Ben-Ezra was the principal researcher in the joint industry-academy project TRANSMOR focused on automatic detection and classification of power transients in WDM optical communication networks. In the period of years 2007-2009, Prof. Yosef Ben-Ezra was the principal researcher in a joint industry-academy project, DIAMOND, that developed high-spectral-efficient modulation techniques for modern optical communications. In the framework of the MAGNET project, Tera-Santa Prof. Ben-Ezra develops the novel method of OFDM based on Multiwavelets. He is currently working on the silicon photonic implementation of the Multiwavelet OFDM in the Peta-Cloud consortium. He has co-authored over 85 papers in international journals and conferences in fields of semiconductor physics and nonlinear effects and optical communication. He is the author 15 chapters in scientific books and of 12 patents.

Abstract:

 Quantum dot (QD) lasers and semiconductor optical amplifiers (QD-SOA) are characterized by the ultrafast carrier dynamics, low threshold bias current, temperature stability, large spectral bandwidth, low energy consumption, and compatibility with modern photonic technologies. They attracted interest as promising candidates for the applications in modern photonics due to these exceptional properties caused by the 3D confinement of the carriers in QDs. We investigated the influence of the carrier dynamics in QD lasers and QD-SOA on their performance as the photonic system components theoretically. We proposed the theoretical model of all-optical processors based on the Mach-Zehnder interferometer (MZI) with QD-SOAs in both its arms, which can perform under different conditions wavelength conversion (WC), logic operations, and optical regeneration. We investigated theoretically different applications of QD lasers and QD-SOAs for radio-over-fiber (RoF) for 5G systems,all-optical memory, and ultra-fast switching for the data centers.

Biography:

Robson Alexandre Brochetti has a degree in Pharmacy and Biochemistry from Universidade Nove de Julho, with an emphasis on Clinical and Toxicological Analysis. Specialization in Clinical and Hospital Pharmacy; Master in Biophotonics applied to Health Sciences area of concentration: Phototherapy and Dosimetry and PhD in Biophotonics applied to Health Sciences area of concentration: Phototherapy and Dosimetry. He is currently a Professor in the Pharmacy undergraduate course at Universidade Nove de Julho - UNINOVE in the areas of Clinical Pharmacy and University Pharmacy (Pharmacy School) and with a research project in the doctorate of asthma resistant to corticosteroids.

Abstract:

Asthma is a chronic inflammatory disease characterized by lung cell recruitment and airway hyperresponsiveness. Treatment consists of bronchodilator antagonists, corticosteroids, or leukotrienes. Photobiomodulation (PBM) appears as a possibility in the treatment of lung diseases, and we focus on evaluating the effects of PBM on lung function in the experimental model of asthma. Male wistar rats were sensitized or challenged with ovalbumin (OVA) and treated or not with PBM (1h and 4h after each challenge with OVA). After 24 h of the last challenge with OVA, lung function and levels and gene expression of nitrites and eicosanoids were evaluated. Wavelength: 660 nm; Power: 160 mW; Power density: 38.5 mW/cm²; Energy density: 5.8 J/cm²; Exposure time: 150 s; 24J energy;The irradiation was performed at a single point, reaching the trachea and lungs. The data showed that PBM in allergic rats caused reduced respiratory resistance (Rrs), respiratory elastance (Ers) to cholinergic stimuli. The effects appear to be dependent on the balance of nitric oxide and eicosanoids since we have demonstrated high levels of nitrites concomitantly with reduced levels of eicosanoids in lung explants. We have also shown increased nitric oxide synthase (NOS) gene expression and decreased gene expression of cyclooxygenase enzymes (COX1 and COX2) in lung tissue after treatment with PBM. The data presented show that treatment with PBM reduced respiratory mechanics during the allergic response, altering the nitric oxide/eicosanoids ratio and opening the possibilities of treating episodes of bronchoconstriction.

Biography:

Andreia La Selva is a dental surgeon, specialist in stomatology and PhD student in biophotonics applied to health sciences at Universidade Nove de Julho, São Paulo, Brazil. His line of research is in photodynamic therapy.

Abstract:

The labialis infections by Herpes Simplex Virus type 1(HSV-1) are contagious and cause discomfort and pain, in addition to being recurrent. The gold standard treatment is acyclovir, but there is viral resistance and does not prevent a recurrence. Antimicrobial photodynamic therapy (aPDT) is a promising approach because, in addition to its excellent topical antiviral effect, it does not induce resistance and prevents recurrence, according to some case reports. This controlled, randomized, double-blind, twelve-month follow-up clinical trial aims to compare the use of aPDT (660nm, 100mW, 120J/cm², 0.005% methylene blue) with topical acyclovir therapy in the treatment of herpetic lesions in stages of vesicles and ulcers through the evaluation of the time to resolve the lesion Two groups: G1- experimental group- aPDT and acyclovir placebo (n= 12) and G2- control group- acyclovir treatment and aPDT placebo (n= 12) will be formed. Secondary variables are pain (visual analogic scale), analysis of cytokines by ELISA (IL1β, IL-6, TNF-α, IL, 10), quantification of HSV-1 by RT-qPCR, and recurrence. The lesions will be evaluated 3 and 7 days after the proposed treatment (treatment effectiveness). Follow-up in month 1, 6 and 12 after intervention (recurrence assessment). A questionnaire will assess the impact of oral health on the participants' quality of life (ohip-14), on the first day and after one year of care. For statistical analysis: ANOVA two-way, complemented by the Bonferroni test. We expect a shorter interval of time for the remission of the disease, without recurrence of the lesion at the site where aPDT was applied.

Biography:

Marlene Ferreira works at Nove de Julho University as a professor in the Health Department. She teaches Anatomy and Physiology to Health Science undergraduate students for the last 5 years. She has a Higher Education Specialization and a Master’s degree in Morphofunctional Sciences from the University of São Paulo in 2014. Her primary research interests are in the field of cellular biology and cancer. Specifically, nowadays, she is interested in oral squamous stem cells and the influence of photodynamic therapy. She is developing her doctoral thesis at Nove de Julho University in Biofotonic Applied to Health Science.

Abstract:

Introduction: Squamous cell carcinoma is the most prevalent malignant neoplasm in the oral cavity. The main therapeutic modalities of OSCC are surgery and radiotherapy alone or in combination with cisplatin. Photodynamic Therapy (PDT) is based on the use of photosensitizers, whose cytotoxicity is activated by light, leading to the death of target cells. Some studies have demonstrated that PDT can be an alternative treatment to OSCC in initial stages. Objective: The aim of this study was to evaluate the effects of PDT on the cellular viability of OSCC cell lines using different dosimetric parameters.  

Material and Methods: CA1 and Luc4 cell lines were cultivated in DMEMF12+10%FBS and supplements. 5x104 cells were plated in 96-well plates and divided into the following groups: control, LED, 5-aminolevulinic acid (5-ALA) and 5-ALA-PDT (5-ALA+LED). Cells in the 5-ALA and PDT groups were incubated with 0.5 mM; 0.75 mM e 1mM 5-ALA at 37ºC for 4h. Next, cells were washed, and the groups LED and PDT were irradiated with a diode emission light (LED) using the BioLambda LedBOX, 660nm, 3J/cm² or 6J/cm², 49.5 mW/cm² and 40% or 80% of power capacity. Cell viability was evaluated after 24h using the MTS and neutral Red assays.

Results: CA1 and Luc4 cell lines showed a significant decrease in cellular viability after 5-ALA-PDT with 6J/cm2, 5-ALA at 1mM, and 80% of the power. No difference in cell viability was observed with 3J/cm² or 6J/cm² and 40 % and 80% of power in both cell lines. In addition, a significant decrease in neutral red incorporation was noticed in PDT groups (1mM 5-ALA, 6J/cm^2, and 80% of power) from CA1 and Luc4 when compared to all groups.

Conclusion: PDT was able to decrease the cellular viability in OSCC cell lines, and further studies are needed to evaluate its effects on other biological processes in OSCC.

Biography:

Bárbara Sampaio Dias Martins Mansano graduated as the best academic performance in Biomedicine at Paulista University (2010-2014) and qualified in Human Histology at São Paulo University (2014), performing sandwich graduation in Biomedical Sciences at Wright State University (2012-2013) by the Science Without Borders program (CAPES and CNPq). After, she obtained her master's degree with two honorable mentions in Biological Sciences (Molecular Biology) at the Federal University of São Paulo (2015-2017) with FAPESP scholarship. Also, she specialized in Teacher Education for Higher Education at Nove de Julho University (2017-2018). Currently, she is doing a Ph.D. in Biophotonics Applied to Health at Nove de Julho University with CAPES scholarship, participating in published studies, and a second-place prize work. Her research interests and experiences are in the fields of Molecular Biology, Biotechnology, Immunology, Biochemistry, Biophotonics, and Histology.

Abstract:

Myocardial infarction is a major cause of morbidity and mortality worldwide. Mesenchymal stem cells (MSCs) are being studied due to the paracrine effects of secreted cytokines and growth factors that decrease inflammation and scar formation and increase local capillarity in infarcted myocardium. However, the loss of MSCs at the transplanted site is large. To address this problem, the use of light sources is a promising tool. In this study, we irradiated MSCs with LEDs seeking positive changes in cell metabolism and enhancing cytokines and growth factors secretion to improve cardiac function later when transplanted into the infarcted heart. Adipocyte derived MSCs were obtained from Fischer-344 male rats (CEUA 5883160218), irradiated once every other day for a week with 630 nm LED box (Biolambda, Brazil) and analyzed the repercussion of different radiant exposures (0.5, 2 and 4 J/cm²). The Control group was kept in the dark for the same time as 4 J/cm²-placebo. Analyses were performed 24 hours after the last irradiation. None of the radiant exposures induced oxidative stress, DNA damage, or alterations in the levels of TNF-α. The 4 J/cm² irradiation up-regulated mitochondrial metabolism, ATP production, and IL-6, IGF-1, and NOx secretion. The 2 J/cm² had no significant action on the MSCs. Besides VEGF levels were significantly higher with 0.5 J/cm², a down-regulation in IL-10 level was detected. Thus, we concluded that the conditioning treatment with 4 J/cm² showed the best results in improving MSCs metabolism and secretion, being a good candidate to perform future cellular therapy in the infarcted myocardium.

Biography:

Bianca Godoy-Miranda is graduated in Dentistry from Universidade Nove de Julho - UNINOVE. She was a monitor of the disciplines of Dental Materials ll and Periodontics I and ll, as well as an advisor to the League of Human Anatomy Milton Picosse Head and Neck and vice president of the Loducca League of Maxillofacial Surgery and Traumatology. She was also a member of the group of Scientific Initiation in Biophotonics from 2014 to 2017. She was a special student of the Master in Biophotonics and participated in the program "Paths for the Master". She is currently a specialist at UNINOVE in Public Health with an emphasis on Family Health and a student of the Master's degree in Biophotonics.

Abstract:

Periodontal disease is an inflammatory response to oral biofilm, and the treatment consists of scaling and root planing. As an adjunct to this treatment, antimicrobial photodynamic therapy (aPDT) has been used, and it consists of the use of photosensitizer (FS) and a light source for the formation of reactive oxygen species. Therefore, the study aimed to evaluate the use of aPDT in Aggregatibacter actinomycentecomitans, as well as the optimization of parameters. For this, A. actinomycentecomitans (ATCC 29523) was used, cultivated in microaerophilia for 48 h. Then, the experiments were carried out in triplicate, with 6 groups: 1) Control, without intervention 2) Light, which was only irradiated 3) FS, where only FS was used 4) PDT1, which was performed aPDT with irradiation of 1min 5 ) PDT3, PDT with 3 min irradiation and 6) PDT5, PDT with 5 min irradiation. Methylene blue (Sigma – Aldrich, USA) was used as a dye at the final concentration of 100 µM and irradiated with laser λ = 660nm (Photon Lase lll, DMC, São Carlos, Brazil) with a power of 100mW and a radiant exposure of 215 J / cm². After microbial growth, the cfu / mL count was performed, and the mean and standard deviation were performed. The control, light and FS groups showed 1x10⁹cfu / mL, and a microbial reduction of 7 orders of magnitude was achieved after 5 min of irradiation. It is concluded that aPDT was effective in microbial inactivation of A. actinomycentecomitans in vitro.

Biography:

Angela Molon graduated in Dentistry at Nove de Julho University, São Paulo, Brazil, in 2017. During her graduation, she worked as a volunteer at the Research Laboratory, focusing on Molecular and Cellular Biology, Immunology, and stem cells. Nowadays, she is a Master’s student at Nove de Julho University in the Postgraduate Program of Biophotonic Applied to Health Science. She is studying the effects of photodynamic therapy and immunotherapy in the treatment of oral squamous cell carcinoma.

Abstract:

The presence of the cancer stem cells (CSC) in oral squamous cell carcinoma (OSCC) has been previously associated with recurrence, resistance to conventional therapy, and poor prognosis. Thus, there is an urgent need to improve the treatment of OSCC, mainly for patients with recurrent or metastatic disease. Photodynamic therapy (PDT) is a minimally invasive therapy able to promote cell death directly, immune surveillance, and disrupts tumor vasculature. Thus, this study evaluated the subpopulations of CSC identified with the phenotype CD44^high/ESA^high and CD44^high/ESA^low in OSCC cell lines as well as the expression of MICA/B (stressed-induced ligand for Natural Killer cells). Ca1 and Luc4 cell lines were divided into control, LED, 5-aminolevulinic acid (5-ALA, 1mM), and 5-ALA-PDT (5-ALA+LED) groups. Cells were irradiated with a diode emission light (LED) using the Bio Lambda Led BOX, 660nm, 6J/cm², 49.5 mW/cm². Cells were incubated for 24h and then collected to evaluate the expression of CD44, ESA, and MICA/B by flow cytometry. The subpopulation of CSC with the phenotype CD44^high/ESA^high (epithelial morphology) was significantly decreased after PDT when compared to control, LED, and 5-ALA groups. However, no difference was observed in the percentage of cells with the phenotype CD44^high/ESA^low (mesenchymal morphology)in all groups. MICA/B expression was induced after PDT. In conclusion, PDT was able to decrease the CD44^high/ESA^high cells and to promote the expression of MICA/B, contributing to eradicate the CSC and probably, to the activation of immune cells.

Biography:

Claudio Teruo Kassa is a Graduate in Dentistry from the State University of Londrina - UEL in 1992. He is a specialist in Periodontics and Orofacial harmonization, qualified in Ozone Therapy, Laser Therapy, and Floral Therapy. Currently attending Post Graduation Program in Biophotonics applied to Health Sciences University Nove de Julho - UNINOVE.

Abstract:

Periodontitis is an inflammatory disease that affects the supportive tissues of the teeth in response to the presence of microorganisms. The gold standard treatment is scaling and root planing. To reduce the use of antibiotics, antimicrobial photodynamic therapy has been studied as an adjunct in periodontal treatment. The main limitation of the technique is the formation of dimers that decrease the effectiveness of the photosensitizer, and sodium dodecyl sulfate has been shown to decrease dimerization. The aim of this study is to evaluate the photodynamic effect mediated by methylene blue in sodium dodecyl sulfate for the adjuvant treatment of periodontitis. This clinical trial will be performed with 40 participants, and all of them will receive scaling and root planing. After 40 days, photodynamic therapy with real irradiation or placebo irradiation will be applied. The random allocation will be in the following groups: 1) group treated with scaling and root planing and photodynamic therapy with methylene blue in sodium dodecyl sulfate; or 2) with photodynamic therapy with methylene blue; 3) and the group treated with root scraping and straightening associated with photosensitizer without light irradiation and 4) treated with a photosensitizer in sodium dodecyl sulfate without light irradiation. The photosensitizer will be in contact for 1 min and the irradiation time or not 2 min. The laser’s wavelength will be 660 nm and 100 mW of power. The primary outcome will be microbial count, and secondary outcomes will be clinical probing depth, clinical attachment level, and bleeding on probing.

Biography:

Daniella Teixeira Bezerra is a pediatric infectious disease specialist and joined, in 2019, the Nove de Julho University as a Ph.D. student in Biophotonics Applied to Health Sciences Program. She has been teaching medicine at the Nove de Julho University, since 2018. Daniella has knowledge of laser therapy, microbiology, and has experience in nosocomial infection control.

Abstract:

Infections are the leading cause of morbidity and mortality among patients with chronic kidney disease (CKD)on dialysis therapy. Staphylococcus aureus is a major agent, and previous nasal colonization represents an independent risk factor for infection. The nasal decolonization strategy reduces the infection rate in this population. The gold standard treatment is topical mupirocin, but there are reports of increasing bacterial resistance. Antimicrobial photodynamic therapy (aPDT) is a promising approach due to its potential bactericidal effect and low tendency to induce resistance.This controlled randomized, blinded, three-months follow-up clinical trial aims to compare the use of aPDT (»660nm, 400mW/cm², 0.01% methylene blue) with mupirocin therapy in nasal decolonization of among patients with CKD on dialysis therapy, through a semi-quantitative microbiological evaluation before and after intervention and recolonization time. Two groups G1- aPDT decolonization (n = 17) and G2- mupirocin treatment (n = 17) will be formed. Secretions from the anterior nasal cavities will be collected - at times T0 (before intervention- carrier status), T1 (first follow-up after intervention - decolonization effectiveness), T2, and T3 (at 1 and 3 months- recolonization). The samples will be sown in an anaerobic culture medium, and bacterial colonies will be identified by mass spectrometry - MALDI-TOF and tested for the antimicrobial sensitivity profile for Staphylococcus aureus(automated method Vitek 2). A questionnaire will be applied to identify possible factors related to colonization in this population. For statistical analysis: ANOVA two-way, complemented by the Bonferroni test. We expect treatments with aPDT and mupirocin to be equivalent.

Biography:

EDUARDO VASQUES DA FONSECA is a Ph.D. student of Post graduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University – UNINOVE, São Paulo, Brazil, Specialist in Surgery and Traumatology Oral Maxillofacial; Coordinator of the Buco Maxillofacial Surgery and Traumatology Service of the Mandaqui Hospital Complex and at Hospital Dr Osirís Florindo Coelho, São Paulo, Brazil.

Abstract:

The proposed study aims to evaluate the efficacy of LED devices regarding the control of pain, edema, paresthesia, and bone repair following surgical maxillary disjunction. The secondary aims are to evaluate the degree of anxiety and the impact of the surgical procedure on quality of life. A randomized, controlled, double-blind clinical trial will be conducted with 72 patients. The procedures will be performed by Three surgeons. Two examiners blinded to the allocation of the patients to the different experimental groups will perform the preoperative and postoperative evaluations, and another researcher will administer PBM. Prior to each surgery, five facial measurements, a periapical radiographic exam, facial and oral sensitivity tests, and evaluation of anxiety (Beck questionnaire and determination of salivary IL-1β, IL-6, TNF-α, and cortisol) will be performed. Immediately after the surgeries, the participants will be randomly allocated to the active and sham PBM groups. In the active group, the participants will receive nine applications of PBM (immediate postoperative period, 1-120 days) with a facial device (57 LEDs at 660 nm and 74 at 850 nm, 5 mW; 6J per point) and an intraoral device (3 LEDs at 660 nm, 5 mW; 2J per point). In the sham group, irradiation will be simulated. Data will be collected in periods up to 120 days after surgery, depending on the variable analyzed. A normality test will be used to determine the appropriate statistical tests for each dataset, with the level of significance set to 5%.

Biography:

Ellen Perim Rosa is a Ph.D. student in Biophotonics Applied to Health Sciences - Nove de Julho University. Specialist in Orthodontics, NAP Odonto, and DDS in Dentistry, UNINOVE. Interest in Biophotonics, Orthodontics, and Periodontics.

Abstract:

Corrective orthodontics is effective in recovering aesthetics and function; nonetheless, factors such as gingivitis contribute to its interruption. Orthodontic patients with gingivitis can be treated in the dental office with scraping; however, areas of gingival growth are difficult to access. Therefore, this study aims to test the effectiveness of antimicrobial photodynamic therapy (aPDT) as an adjunct in the decontamination of these areas. aPDT associates the use of a photosensitizer, light and oxygen  to promote an antimicrobial effect. In this clinical, randomized, and double-blind trial, 34 participants in orthodontic treatment, presenting gingivitis and gingival growth, will be included, divided into 1. Control group- Ultrasound scraping + aPDT placebo and 2. Experimental group- Ultrasound scraping + aPDT. aPDT parameters will be: methylene blue 0.005%, λ = 660nm, 9J per site, irradiance = 3.5W/cm, radiant exposure = 318J/cm2. The analyzes will take place at baseline, 7 and 21 days after treatment. Clinical parameters will include a gingival index, plaque index, and probing depth. The crevicular fluid will be collected from 4 sites for analysis of IL-6 and IL-10 cytokines, by ELISA. The test for parametric data will be one-way ANOVA and for non-parametric Mann Whitney. Data will be presented by means ± SD and p defined at 0.05. The results of this study may present therapeutic alternatives. It is expected that there will be a decrease in IL-6 and an increase in IL-10, as well as an improvement in the clinical parameters of sites treated with aPDT and scaling.

Biography:

Gabriela Benedito is a Ph.D. student in Biophotonics applied to Health Sciences – UNINOVE. Specialization in University education UNINOVE; Master's degree in Biophotonics applied to health sciences (UNINOVE 2017-2019); Bachelor's degree in Chemistry (UNINOVE 2014-2016). Volunteer professor in the PIBID Project (2016), Volunteer Professor in the Mafalda Pre-vestibular course in Organic Chemistry (2015). She has experience in the following topics: Photobiomodulation - Mechanisms and Applications, Interaction of reactive species with biological systems: skin cell culture. Cutaneous photoaging, use of natural assets for photoaging. Experience in the methods: Cell Culture, Genica expression, lipid peroxidation, cell viability, among others.

Abstract:

Skin photoaging is induced by ultraviolet radiation that leads to the formation of reactive oxygen species (EROS). Vegetal extracts present high antioxidant capacity due to polyphenols content. They act as reducing the concentration of the oxidizing species in the tissue and reducing inflammation, among others. Photobiomodulation (PBM) is a technique known by inducing cellular activity, ATP levels, modulating inflammation, etc. The combination of the antioxidant capacity of vegetal extracts and PBM seems to be a promising combination in order to reduce the harmful effects of UVA radiation. The objective of this work was to evaluate the catalase gene expression in human keratinocytes exposed to UVA after receiving vegetal extracts and PBM. For this, human keratinocytes (HaCaT) were seeded in 35mm well plates (150,000 cells/plate) and, after attachment was exposed to UVA (366 ± 10 nm, 2.5 mW / cm², 5400 sec). Then, treated with Chamomile (Chamomilla recutita Flower Extract) and Fig (Ficus carica Fruit Extract) extract at 0.3% for 24 hours and, finally, PBM (640 ± 12 nm, 2.6 mW/cm², 420 sec). At the end of the treatments, cells were kept in the incubator for 24 hours. RNA was extracted, and catalase gene expression was detected by quantitative PCR. In the dark, Chamomile increased catalase gene expression showing a photoprotective potential. UVA reduces the gene expression, while Chamomile use combined to PBM, showed an increased gene expression in relation to the UVA group. Fig extract does not alter the catalase gene expression in any treatment.

Biography:

Jefferson André Pires is a physician, and general surgeon who graduated from the Federal University of Pelotas-RS, Brazil, graduating from the Ph.D. program in Biophotonics from the UNINOVE, São Paulo, Brazil and is also a resident in Plastic Surgery at the Conjunto Hospitalar do Mandaqui, São Paulo, Brazil.

Abstract:

Keloids are fibroproliferative scar difficult to treat and with high taxes of recurrence after treatments. The keloids etiopathogenesis is related to the disordered fibroblasts growth and an increase of the transforming growth factor-beta (TGF-β) levels. The main intervention is the surgical removal associated with the injection of corticosteroids. The photobiomodulation (PBM) with blue light has demonstrated in in vitro studies an inhibition of fibroblasts and TGF-β levels. The present study is a randomized, controlled, and double-blind clinical trial which aim is to verify the effects of PBM associated with the corticoid treatment during the pre and postoperative periods of keloid removal (keloids of any part of the body sutured primarily after the surgery). Participants (N=38) will be randomly allocated into two groups named: (1)Sham group (N=19) submitted to the standard procedure (corticoid + removal surgery) and (2) PBM group(N=19)  (PMB + Corticoid + removal surgery)using blue light (λ=470nm; P=400mW; E=24J, per point being 1 point per linear scar centimeter). The rate of recurrence and the quality of the new scars will be analyzed using the Vancouver scar scale. Silicone molds of keloids and new scars will be made and transposed in the acrylic surface to obtain measurements using optical coherence tomography. The histopathology of the resected keloid will also be analyzed to verify the fibroblasts, TGF-β, and collagen deposition and organization. Questionnaires (Qualifibro-UNIFESP and PSAQ) to assess the participant's quality of life and scar will be performed. The data will be submitted to statistical analysis.

Biography:

José Marcelo is currently a Ph.D. student in Biophotonics Applied to Health Sciences, UNINOVE. He graduated in Nursing at UNINOVE, where he initiated his studies (scientific initiation) and got a master's degree. His primary objectives in research are related to photodynamic therapy applied in cell culture and/or microorganisms.

Abstract:

Methylene Blue (MB) is a compound that has been widely investigated as an antimicrobial agent for Photodynamic Therapy (PDT). Recently, our research group showed that the control of aggregation, through the association of MB with sodium dodecyl sulfate (SDS) potentiates the PDT action. However, in addition to optimizing the vehicle used, dosimetry also plays an important role in the outcome of therapy. The objective of this study is to compare the dosimetry of MB mediated PDT both in water and associated with SDS in Candida albicans. The fungi will be placed in two 48-well plates with their treatment (control; MB aqueous solution; SDS 0.25%; MB + SDS 0.25%). Then, the samples will be homogenized and incubated in the dark for 5 minutes. After incubation, one plate will be the plate that has been exposed to light (659 ± 9nm, in different dosimetry, i.e., keeping the irradiance constant and varying radiant exposure; later, doing the opposite). At the end of the irradiation, the samples will be diluted in ultra-pure water (10to 100000 times). The dilutions will be spread on sabouraud dextrose agar and placed in an incubator at 37°C for 24 hours. After this period, colonies will be counted. This work will show the most important dosimetric parameter (irradiance or radiant exposure) for antimicrobial PDT.

Biography:

Marcelo Marreira is a nurse specialist in obstetric and gynecological nursing at the Abc School of Medicine. Master in Biophotonics Applied to Health Sciences by Nove de JulhoUniversity and Ph.D. student in the same department. He is a professor in the nursing department at Nove de Julho University.

Abstract:

The search for non-invasive aesthetic procedures for localized fat reduction has gained attention. Some studies have shown that Photobiomodulation (PBM) can be used in body contouring. However, there is no standardization of the protocol. The objective of this study will be to compare the different light wavelengths when using PBM as a technique for the reduction of abdominal waist circumference. 174 patients will be recruited, and then divided into 3 groups: RED PBM; INFRARED PBM; and PLACEBO (Sham). The treatments will consist of eight sessions, twice a week, for four weeks. At each session, the participants will receive 30 minutes PBM (127 J/cm²), and further, both groups will receive 30 min Aussie Current (4kHz, modulated at 10Hz, 40-60mA). The main outcome of this study will be waist circumference reduction. The secondary variables will be anthropometric data, lipid profile, liver function, changes in the local microcirculation, and the quality of life and self-esteem. The analyses will be performed at four stages of the research, D0, end of the eighth session, 15 days after the last session, and 180 days after the last session. This work will show the efficacy and the best wavelength for PBM when using for waist reduction. Since it will be expected that PBM release of triglycerides from adipocytes, Aussie current will act on the increase of b-oxidation. The control of the lipid profile is important since the availability of triacylglycerol may affect the lipid profile in the blood, bringing consequences for the general health of the individual.

Biography:

Michelle M. Sena is a biomedical who joined as a Ph.D. student in the Postgraduate Program in Biophotonics Applied to Health Sciences at Universidade Nove de Julho (UNINOVE). Prior to attending UNINOVE, she was a master’s student in Experimental Pathology at the State University of Londrina and obtained a degree in Aesthetic Biomedicine postgraduate course. Her primary research interests are related to the use of photobiomodulation in the treatment of aesthetic dysfunctions, specifically in the determination of dosimetric parameters that can be applied in clinical practice.

Abstract:

The current routine that includes stress and sedentary lifestyle, alcohol consumption, smoking, poor eating habits, and low sleep quality, when combined with genetic factors, are directly related to overweight and fat deposits formation. This interferes with self-esteem and quality of life for a large part of the world population. Aesthetic resources destined for localized fat treatment, such as photobiomodulation and low-frequency ultrasound, although seem promising, have low scientific support for the combined use. This study will aim to compare the effects of photobiomodulation, low-frequency ultrasound, and the association of these techniques in abdominal localized fat. The main outcome will be abdominal skinfold thickness. Secondary outcomes will include anthropometric parameters, the temperature of the treated region, and biochemical and liver enzymatic markers will be evaluated. Visual changes due to treatments will also be assessed through photographic records. Finally, the subjective assessment will be collected through questionnaires that measure the quality of life (WHOQOL-BREF), the body self-image scale (BSQ-34), and the degree of patient satisfaction. The mechanism by which photobiomodulation acts in lipolysis is related to the production of reactive oxygen species that interact with the plasma membrane through lipid peroxidation, while low-frequency ultrasound seems to destabilize the membrane due to cavitation. Thus, both are involved on release of triglycerides present in adipocyte deposits for interstitial space, so it is expected that the combination of these techniques will lead to a greater reduction in the abdominal skinfold when compared to the isolated application of each technique.

Biography:

Pamella Motta is a dental surgeon, a specialist in pediatric dentistry. She works in the field of facial aesthetics. She joined Nove de Julho University to pursue a master's degree in biophotonics. She developed an interest in the area because she already worked as an assistant to Ph.D. Sandra Kalil Bussadori, where she started using laser and led in treatments and developed an interest in research. Following the team's line of research, she conducts studies on halitosis. Pamella is also very interested in the use of laser in facial aesthetics. She really likes to travel and always tries to associate travel with studies.

Abstract:

Halitosis is a term that defines any foul odor emanating from the oral cavity. The origin may be local or systemic. The proposed project aims to determine whether treatment with antimicrobial photodynamic therapy (aPDT) and treatment with probiotics are effective at eliminating halitosis. 52 patients from 18 to 25 years old with a diagnosis of halitosis (H₂S ≥ 112 ppb determined by gas chromatography) will be randomly allocated to four groups (n=13) who will receive different treatments: Group 1 – treatment with teeth brushing, dental floss and tongue scraper; Group 2 – brushing, dental floss and aPDT; Group 3 – brushing, dental floss, and probiotics; Group 4 – brushing, flossing, aPDT, and probiotics. The results of the halimeter testing will be compared before, immediately after, seven days and thirty days after treatment. The microbiological analysis of the coated tongue will be performed at these same times. The quantitative analysis will be conducted using real-time PCRq. The normality of the data will be determined using the Shapiro-Wilk test. Data with normal distribution will be analyzed using analysis of variance (ANOVA). Non-parametric data will be analyzed using the Kruskal-Wallis test. The Wilcoxon test will be used to analyze the results of each treatment at the different evaluation periods. This study has been approved by the Ethics Committee of UNINOVE under process number 3.669.442. Results will be published in peer-reviewed journals and will be presented at national and international conferences.

Biography:

Marcos Momolli is a doctoral student in the Biophotonics Department at Nove de Julho University (UNINOVE), São Paulo, Brazil. He is a general surgeon graduated at the Hospital de Clínicas de Porto Alegre (HCPA) and is attending the last year of his residency in plastic surgery at Conjunto Hospitalar do Mandaqui (CHM) in São Paulo. He is also a fellow student in Cosmiatry at the Boggio Institute in São Paulo. His main research interests are in the field of photobiomodulation for skin rejuvenation and tissue repair, which are important factors in his clinical practice.

Abstract:

Photodamage is a growing concern in contemporary society because it promotes early skin aging and different pathologies resulting from prolonged and repeated exposure to ultraviolet solar radiation, which is considered the main extrinsic factor of this process. Affected by radiation, the hands play an important role in the manifestation and visibility of aging, as they are very exposed body regions. Knowing this, a holistic body approach to rejuvenation is necessary, and hand treatment cannot be overlooked. This concern results in a growing rise in minimally invasive rejuvenation treatments capable of providing satisfactory results such as chemical peel and photobiomodulation. The study proposal is developed by the potential mechanism of tissue repair, skin rejuvenation, anti-inflammatory, and analgesic effects of photobiomodulation, complementing the benefits of chemical peel. Therefore, this randomized, controlled, double-blind clinical trial aims to compare the effects of monotherapy with 20% trichloroacetic acid (TCA) peel and the effects of the association of 20% TCA peel with 660nm LED photobiomodulation in photoaging treatment of hands. To achieve this, participants will be divided into two groups of different treatments and the results compared at the end of 04 sessions. Features of photoaging will be analyzed, such as fine and coarse wrinkles, abnormal pigmentation, and an overall assessment of the back of the hands through periodic standardized photographs. A visual analog pain scale and a 5-point Likert scale to assess participants’ satisfaction will also be applied at the end of the study.

Biography:

Silvana Torres Perez is a Ph.D. student in the Biophotonics Applied to Health Sciences Postgraduate Program, Nove de Julho University - UNINOVE, São Paulo, Brazil, Graduated in Medicine, did Medical Residency in General Surgery and Specialization in Angiology and Vascular Surgery and has a master's degree in Biophotonics Applied to Health Sciences.

Abstract:

Peripheral arterial occlusive disease (PAOD) is a late manifestation of atherosclerosis in the lower limbs due to a progressive decrease in blood supplying the tissues. Current treatments for PAOD are not so effective in the long term, and many patients progress to severe ischemia, running the risk of amputation. Photobiomodulation (PBM) has a proven effect on angiogenesis when using red and infrared sources. This project aims to evaluate the effect of PBM on angiogenesis (growth and proliferation of new blood vessels from existing vascular structures) and arteriogenesis (proliferation of pre-existing collateral arteries) using a lower limb ischemia model in rats. Visualization and measurement of the vessels will be performed via microscopy. 30 adult Wistar rats, under general anesthesia, will be submitted to thermography and oximetry of the hind legs, followed by surgical interruption of the femoral artery, causing ischemia. The animals will be divided into 2 groups (control and PBM). The control group will not receive any treatment. Irradiation with red (660 nm) and infrared (850 nm) laser, the total energy of 4 J / point every cm, will be applied 5x / week to the PBM group; the application will be along the femoral artery path, from the immediate postoperative period until the end of the experiment (30 days). Capillary density will be analyzed based on histological sections of the gastrocnemius muscle and measurement of collateral vessel density at 7, 14, and 30 days. The results will be treated statistically.

Biography:

Thalita Molinos Campos is a dentist, graduated from Universidade Nove de Julho (UNINOVE). When she graduated, she won the João Sampaio Doria award, from the Regional Council of Dentistry, as the best student in the university's dentistry course. She did a residency in primary health care and family health, working with health promotion and prevention projects aimed at the population in social vulnerability. She attended a master's degree in Biophotonics Applied to Health Sciences also at UNINOVE, with works focused on photobiomodulation in oral mucositis and breast cleft. Today she is pursuing a doctorate within the same program. She is a collaborating professor in the dentistry course, within the disciplines of the health promotion nucleus, at the same university.

Abstract:

One of the most performed procedures in dentistry is the extraction of third molars, a surgery that causes a lot of discomfort to patients, with pain, edema, and trismus due to surgical trauma. To reduce these negative post-surgical effects, anti-inflammatory drugs are prescribed after extraction, but these drugs can have unwanted effects. Photobiomodulation is a technique to modulate inflammation, accelerate tissue repair, and also reduce pain and discomfort in different clinical situations, using low-level laser therapy and LED (light-emitting diode). Thus, the present study aims to carry out a Systematic Review of extraction in third molars with photobiomodulation, followed by a meta-analysis to assess cost-effectiveness. For the systematic review and meta-analysis, studies published between 2005 and 2020 will be selected. Searches will be conducted through the international databases PubMed, Web of Science, and MEDLINE. It will be used the search terms "third molar", "phototherapy"," dental extraction", "laser therapy", "third molar extraction", "photobiomodulation"," third mandibular molar", "third molar surgery", "low level laser therapy" and "wisdom tooth removal". The cost-effectiveness establishes whether or not a treatment should be implemented as a therapeutic measure, is calculated by the difference between the cost of two interventions proposed as treatment divided by the difference between its consequences (effectiveness). The costs will be based on the values with the treatment with the laser and with the use of anti-inflammatory, having as outcome measure pain (visual analog scale) and trismus (mouth opening-mm).

This protocol is registered in PROSPERO CRD42018105658.

Biography:

Maria Lucia Zarvos Varellis, Surgeon Dentist graduate from University of São Paulo, Specialist in Dentistry for Handicaps and Special Needs since 2002, PAHO consultant and Ministry of Health 2010-2011, Enable for use the Laser in Dentistry by SOBRALO and Federal Council of Dentistry, Enable for Hospitalar Dentistry by the Federal Council of Dentistry, Currently attending Post Graduation Program in Biophotonics applied to Health Sciences University Nove de Julho – UNINOVE. She is a professor and has been teaching Dentistry at the University Nove de Julho - UNINOVE since 2016, Line of research is in Photobiomodulation in salivary glands in patients with xerostomia induced by antihypertensive drugs, Participates in the training of undergraduate students for laboratory research.

Abstract:

Biography:

Adriana Mazzoni is a master's student in the Biophotonic' s Program applied to Health Sciences at Universidade Nove de Julho in São Paulo, Brazil. She has been a specialist in pediatric dentistry at the University of Santo Amaro in São Paulo since 1994, has been a Trainer in the Evaluation of the tongue brake by the Ministry of Health of Brazil since 2018, has been a teacher at the Essekabe School of Dentistry in São Paulo since 2018. She studies and works with breastfeeding since 1995, and is very interested in studying in detail the whole subject related to this theme.

Abstract:

Ankyloglossia is an anomaly that is characterized by an abnormally short or thick lingual frenulum that can restrict the movements of the tongue and has been identified as one of the factors that can negatively interfere with breastfeeding, decreasing the ability of the newborn to handle proper. The repositioning of the lingual frenulum, also called lingual frenotomy, is the most recommended for infants because it is a less invasive technique. This surgery can be performed conventionally with a scalpel blade or with thermal resources such as an electric scalpel or high-powered lasers.
Participants in this study will be infants between 0 to 3 months of age diagnosed with ankyloglossia, difficulties during breastfeeding, need for lingual frenotomy and will be randomly distributed into groups and divided into groups: Surgery group with electrocautery and Laser surgery group high power diode.

The objective of the study will be to evaluate the release of the lingual frenulum through the lingual frenotomy performed with a high power diode laser or electrocautery techniques.

Biography:

Rafael Zaratin Beltramin is a dental surgeon, a master's degree student in Rehabilitation Sciences from Universidade Nove de Julho, a specialist in Public Health from Universidade Nove de Julho and graduated in Dentistry from Universidade Nove de Julho. Rafael Zaratin Beltramin has improvement and complementary courses in the areas of bruxism, minor oral surgery, dental aesthetics, botulinum toxin, and facial fillers.

Abstract:

The aim of this systematic review will be to analyze whether children with cerebral palsy (CP) have abnormal bilateral masseter and temporal muscle activation during mastication. This review will follow the recommendations of the Cochrane Handbook for Systematic Reviews of Interventions and PRISMA statement and will be registered at the PROSPERO platform. A comprehensive search of the literature will be performed using an electronic search (MEDLINE, Embase, Cochrane Library, LILACS, BBO, Clinicaltrials.gov, and WHO/ICTRP) with no restriction regarding date and language. The grey literature will also be screened via OpenGrey. We will include randomized (RCT) comparative observational studies that assessed as primary outcome masticatory muscles (temporal and masseter) activation, by using electromyography parameters such as time of activation, duration, masticatory cycle, and force peak. Two authors will independently select the references retrieved by search strategy using the software Rayyan and will extract the data from included studies. All discordance will be solved by a third author. The risk of bias assessment will be performed using the Newcastle-Ottawa scale. We will calculate the mean differences for continuous outcomes and risk ratios for dichotomous outcomes (CI 95%). If possible, electromyography parameters will be combined in a meta-analysis using the random-effect model in the Review Manager 5.4 software. Heterogeneity between studies will be evaluated. The certainty of the body of the evidence will be assessed using the GRADE approach.

Biography:

Erick Frank Bragato is Ph.D. student of the Postgraduation Program in Biophotonics Applied to Health Sciences, Nove de Julho University – UNINOVE, São Paulo, Brazil. Graduated in medicine in 2014 from the State University of Londrina, Brazil, has a medical residency in General Surgery and is completing his residency in Plastic Surgery in São Paulo, Brazil.

Abstract:

Skin aging is an irreversible, slow, and progressive process, being mainly influenced by age, but also by external factors, such as ultraviolet radiation, smoking, alcohol, among others. Studies have shown the benefits of photobiomodulation (PBM) for facial rejuvenation, especially with the use of the red LED. However, there is still a high level of variability in the treatment parameters and the frequency of application of FBM. The purpose of this study is to compare the effects of PBM with LED mask (660nm, 6.4 mW/cm², 2.67 J/cm²) on facial rejuvenation using 2 application frequencies: one group will receive 2 applications a week for 4 weeks, and the other group will receive 3 applications a week for the same period. After 30 and 90 days, the depth and length of the wrinkles (evaluation of facial impressions by optical coherence tomography); the viscoelasticity of the skin; the evaluations of photographic images by experts (Wrinkle Assessment Scale) and the level of satisfaction of the participants (FACE-Q) will be compared with the data collected before the beginning of the study. All data will be analyzed statistically according to their distribution, seeking a level of statistical significance of 5%.

Biography:

Monise Mendes Rocha is a dental surgeon, a master's degree student in Rehabilitation Sciences from Universidade Nove de Julho, a specialist in Public Health from Universidade Nove de Julho, and graduated in Dentistry from Universidade Nove de Julho. Monise Mendes Rocha has improvement and complementary courses in the areas of bruxism, minor oral surgery, dental aesthetics, botulinum toxin, and facial fillers.

Abstract:

The aims of this systematic review will be to evaluate the effects (benefits and harms) of photobiomodulation for treating masticatory muscle spasticity in children with cerebral palsy. This review will follow the recommendations of the Cochrane Handbook for Systematic Reviews of Interventions and PRISMA statement and will be registered at the PROSPERO platform. A comprehensive search of the literature will be performed using an electronic search (MEDLINE, Embase, Cochrane Library, LILACS, BBO, Clinicaltrials.gov, and WHO/ICTRP) with no restriction regarding date and language. The grey literature will also be screened via OpenGrey. We will consider randomized (RCT) and non-randomized clinical trials (NRCT), that assessed the use of photobiomodulation compared with placebo, no intervention, or another active intervention. Primary outcomes will be muscle spasticity relief, pain intensity, and adverse events. Secondary outcomes will be muscle fatigue, the amplitude of mouth opening, and tooth wear prevention. Two authors will independently select the references retrieved by search strategy using the software Rayyan and will extract the data from included studies. All discordance will be solved by a third author. The risk of bias assessment will be performed using the RoB tool for RCTs and ROBINS-I for NRCT. For the treatment effects estimative, we will calculate mean differences for continuous outcomes and risk ratios for dichotomous outcomes (CI 95%). When possible, treatment effects will be combined in a meta-analysis using the random-effect model in the Review Manager 5.4 software. Heterogeneity between studies will be explored. The certainty of the evidence will be assessed using the GRADE approach.

Biography:

Felipe Murakami Malaquias da Silva graduated in Dentistry from the University of Dentistry, University of São Paulo (FOUSP). Specialist in Orthodontics, Ortogeo - Faculdade Sete Lagoas / São José dos Campos, SP - Brazil. Master and Ph.D. student in Biophotonics Applied to Health Sciences - University Nove de Julho. Presented works at congresses, including at WALT-2018 (Nice, France). His line of research focuses on the use of lasers and their cellular repercussions.

Abstract:

With the loss of teeth, the adjacent tooth moves towards the space left, causes aesthetic and functional problems. The skeletal anchoring system, as mini-implant (MI), has been increasingly used to replace the adjacent tooth to the correct place. Some photobiomodulation (PBM) protocols have been shown to accelerate orthodontic movements, with analgesia and inflammatory modulation. This study will evaluate how PBM interferes with molars verticalization movement, pain, and periodontium's inflammation. A selection of 34 patients with an inclined molar were randomly divided into 2 groups: G1 - verticalization with MI + PBM simulation; G2 - verticalization with MI + PBM. The PBM protocol will be: power of 100 mW, λ 808 nm, 1J per point, 10 points, for 10s, radiant exposure of 25 J / cm². Each 30 days, an orthodontic force will be applied, and PBM will be done at 0, 3, and 7 days, every month, for 3 months. By ELISA, the cytokines IL1β, IL-6, IL-8, IL-10, and TNF-α from the crevicular gingival fluid will be analyzed. We will use an initial and final panoramic radiography to measure the tilt speed. A Visual Analogue Scale and a count of painkillers will be used to assess the laser's analog effect. OHIP-14 questionnaire will be applied to identify the impact of oral health on the participant's quality of life. The ANOVA-one way test will be applied, with a mean ± SD and p-value <0.05. We expected an increase at inclination speed, a decrease in the amount of inflammatory cytokines, and an increase in anti-inflammatory.

Biography:

Juliana Terra Fernandes is a master's student in "Biofôtonia applied to health sciences" at Nove De Julho University. Graduated in Dentistry in 2001, from the University of Mogi Das Cruzes. Specialist in Public Health, at São Leopoldo Mandic College, Specialist in preceptor ship at Hospital Sírio Libanês. She has been working in basic public health for 17 years. She acts as a dental surgeon in the city of Mogi das Cruzes. Her goal is to bring new ideas and technologies to the area of dentistry, focused mainly on public health.

Abstract:

Dental Caries disease is controlled through biofilm control, which balances demineralization and remineralization reactions, considering individual factors to prevent a recurrence. However, there are still few studies that make it possible to systematize a new protocol for the evaluation of dental biofilm through the use of light for the implantation of new biofilm control protocols through the use of optical fluorescence diagnosis. Fluorescence appears clearly in the plaque in red tones, showing the presence of microorganisms in regions where there is biofilm accumulation. 

Objectives: To evaluate the applicability and effectiveness of the diagnosis of oral biofilm with the optical fluorescence technique using the EVINCE (LED with emission centered at 400 ± 10nm - MMOptics, Brazil) equipment and to create a clinical evaluation protocol for the presence of biofilm using the same equipment. Furthermore, we intend to compare the efficacy of the optical fluorescence diagnostic method with the traditional method (Fuchsin-based dye biofilm) and to observe whether EVINCE alone would be sufficient for the evaluation of dental biofilm in young people with mixed dentition without use of traditional dyes. 

Methods: Children (6 to 12 years) will be evaluated by 3 different professionals, starting the evaluation where the 1st evaluator will note the IHOS observing only with EVINCE. In the second stage, a 2nd evaluator will perform the traditional disclosure technique with Fucsina, later noting the IHOS, and finally, 3rd evaluators who will observe with EVINCE the teeth previously stained in stage 2. We expect to find advantages with EVINCE. 

Acknowledgement: This study was financed in party by the “Coordenação de aperfeiçoamento de pessoal de Nível superior-Brasil (Capes) –Finance Code 001”

Biography:

Marina Bertoni Guerra is a doctorate student in Biophotonics Program applied to Health Sciences at Nove de Julho University, São Paulo, Brazil. She is a gynecologist and obstetrician, graduated from the Hospital de Clínicas de Porto Alegre and her subspecialty is in the area of Fetal Medicine. She currently works as a gynecologist and obstetrician in the city of São Paulo. Her primary research interests are in the field of women’s health.

Abstract:

Menopause is a physiological event and is defined as the loss of ovarian follicular activity, with the consequent permanent cessation of menstrual cycles. Its diagnosis is made retrospectively after 12 months of amenorrhea, with no pathological cause involved. This period is marked by several changes in the female organism, mainly the genitourinary syndrome (GUS), which is a collection of signs and symptoms resulting from the state of hypoestrogenism. Almost half of the postmenopausal women will experience symptoms of GUS, with vaginal dryness being the most prevalent, followed by dyspareunia and vulvovaginal irritation. The aim of the study is to evaluate the effect of photobiomodulation with red LED (light-emitting diode) on the symptoms of vulvovaginal atrophy in postmenopausal women. A randomized and controlled clinical trial will be developed, which will include postmenopausal women with signs and symptoms of vulvovaginal atrophy. Participants will be allocated into two groups: those who will undergo photobiomodulation with intravaginal LED, and those who will receive vaginal cream with estriol. Objective and subjective improvement of atrophy will be assessed using the Vaginal Health Index and the visual analog scale, respectively. Vaginal pH, cell maturation index, and changes in sexual function through the Female Sexual Function Index questionnaire will also be assessed. The variables will be analyzed at the time of admission, in one and three months after the intervention.

Biography:

Tânia Oppido Schalch is a dentist, specialist in periodontology and joined, in 2019, the Nove de Julho University as a Master´s student in Biophotonics applied to health sciences program, collaborating in the development of many projects. Attended college at São Paulo University (USP), where she conducted, for 5 years, research in the area of oral microbiology. Tânia has knowledge about laser therapy, microbiology, periodontology, and has experience with clinical and laboratory research.

Abstract:

Pericoronitis is a prevalent condition during the eruption of third molars, many times debilitating. The most indicated treatment (initial phase) is the irrigation. There is no adequate treatment at this phase, and evolution of disease may require antibiotic therapy. To reduce systemic dissemination of infection and antibiotics use, it is important to test the efficiency of treatments in the initial phase of pericoronitis. Antimicrobial photodynamic therapy (aPDT) is an interesting alternative because it is easy to perform and does not cause bacterial resistance. The aim of this study is to evaluate the effectiveness of aPDT in pericoronitis in its initial phase. In this controlled clinical trial, 34 healthy young individuals with pericoronitis will be randomized into the positive control group  (n = 17): irrigation with sterile saline and aPDT (methylene blue-MB, 0.005%, λ = 660 nm ^, 9J per point,  318 J/cm²) and experimental group (n = 17): treatment identical to G1, however, MB will be delivered in a new formulation for oral use. Microbiological quantification will be performed for Tannarella forsythia by PCRq. Saliva and gingival crevicular fluid will be collected to evaluate cytokines. Also, it will be analyzed the pain, edema, and buccal opening and the impact of oral health on quality of life. The variables will be evaluated in the baseline and 4th day after aPDT. For statistical analysis: ANOVA two-way, complemented by the Bonferroni test. We expect that aPDT will improve clinical parameters and reduce the amount of T.forsythia.

Biography:

Alexandre Morais has been a university professor at UNINOVE for 4 years, but he has been teaching for over 14 years. He finished his degree in Dentistry in 1997, obtained his Master's degree in 2010, and is a Ph.D. student at UNINOVE. He is also an international consultant and speaker for a dental products company based in Germany. He has been in clinical practice since 1997 with an emphasis on cosmetic dentistry.

Abstract:

With the increase in aesthetic requirements in Dentistry, alternatives were sought for the elimination of metallic structures. Ceramics are the materials of choice for dental prosthetic rehabilitation because they have many enviable properties. Among them are: aesthetics, abrasion resistance, biocompatibility, little plaque accumulation, low thermal conductivity, and color stability. Zirconia structures are widely used. However, the great disadvantage of zirconia is the low potential for adhesion to resin cement. Unlike conventional porcelains where the vitreous phase can be conditioned by hydrofluoric acid, creating mechanical retentions on the internal surface of the piece, zirconia has a high crystalline content without any vitreous phase at the edges of the 'crystalline organs.' Thus, it is inert to acid conditioning, and its wettability with the cementing (bonding) agent is impaired. In an attempt to increase the mechanical retention between zirconia and cement, some treatments are suggested by the literature: Sandblasting with aluminum oxide and high power lasers. Although there is much information about the effects of this irradiation on enamel and dentin, little is known about the irradiation of this laser as a surface treatment for high-strength dental ceramics. The aim of this work will be to compare the surface roughness and morphological characteristics of Zirconia ceramic surfaces irradiated with Er: YAG laser with the variation of radiant energy.

Biography:

Andréia Martinelli de Siqueira Araujo was graduated in physical therapy by the Nove de Julho University. During the undergraduate period, she participated in a scientific initiation project, which allowed her to know the research better. After she obtained her Master's Degree in Rehabilitation Sciences Post Graduate Program at Nove de Julho University, and she is currently a Ph.D. student in the same Post Graduate program. The main objective of her research area is to evaluate the plasticity of the skeletal muscle.

Abstract:

Skeletal muscle is a tissue with high adaptive capacity altering its characteristics to meet its diverse functional demands. Compensatory hypertrophy (CH) occurs due to excessive mechanical load on a muscle, promoting an increase in the size of muscle fibers. Photobiomodulation (PBM) has demonstrated beneficial effects on muscle tissue during CH. This study aimed to evaluate the effect of PBM on the inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) during the process of CH. Wistar rats were divided into three groups: control group (n=5), hypertrophy (H) group (n = 10) and Hypertrophy + PBM group (n = 10). CH was induced through the ablation of synergist muscles of the plantaris muscle. The preserved plantaris muscle below the removed muscles was submitted to excessive functional load. PBM was performed with low-level laser (AsGaAl, λ = 780 nm; 40 mW; energy density:10 J/cm²; 10 seconds on each point, 8 points; 3.2 J). Animals were euthanized after seven and 14 days. The plantaris muscles were removed and sent for analysis of the gene expression of IL-6 and TNF-α using qPCR. The gene expression results demonstrated an increase in IL-6 in the H+PBM group in comparison to the H group at 14 days as well as an increase in TNF-α in the H+PBM group in comparison to the H group at seven days. Based on findings in the present study, it is concluded that PBM was able to modulate pro-inflammatory cytokines that are essential for the compensatory hypertrophy process.

Biography:

Will be updated soon.

Abstract:

Oral Lichen Planus (OLP) is a chronic mucocutaneous inflammatory autoimmune disease. The lesions may be asymptomatic or have severe pain. The standard treatment consists of using topical and systemic corticosteroids, which are associated with clinical improvement, although there may be side effects or a lack of response to the therapy. Photobiomodulation (FBM) is effective in the treatment of inflammatory diseases, reducing the release of inflammatory cytokines, promoting analgesic effect, and accelerating the repair process. There are no studies in the literature that evaluated the effects of FBM on the modulation of inflammatory cytokines in OLP. Thus, this work aims to evaluate the concentration of TNF-α, IL-17, IL-1β in the serum of patients with OLP, treated with FBM, when compared to conventional treatment. Patients diagnosed with OLP were randomized into two groups (control and FBM). The control group patients were treated with topical 0.05% clobetasol propionate, 3 times a day for 4 weeks, and with laser equipment off, twice a week. In the FBM group, the irradiation was performed twice a week with low power In GaAIP diode laser (680 ± 20nm, power 100mW, energy density 177J / cm 2, 5 seconds, 0.5J total energy per point) for 4 consecutive weeks and with a placebo gel, 3 times a day for 4 weeks to mask the treatment. Peripheral blood was collected at baseline (D0) and at the end of treatment (D30). After processing, the plasma will be aliquoted for the evaluation of cytokines by ELISA.

Biography:

Erika da Silva Mello is a graduate student in Biophotonics at Universidade Nove de Julho. She had a major in Dentistry at Universidade Paulista in 1993, she dedicated herself to general practice with an emphasis on orofacial pain and temporomandibular joint disorders, having completed this specialty in 2009 at Universidade São Marcos. She is also a specialist in acupuncture by the Escola de Terapias Orientales de São Paulo in 2012 and qualified in the clinical use of laser by the University of São Paulo (2017) in the dental clinic, includes training in laser high power and experience in functional rehabilitation. Her main interest is in the field of research in Biophotonics associated with the patient's quality of life.

Abstract:

Preconditioning the tissue offers good results in preventing mucositis in cancer treatments and in preventing muscle injuries in fatigue protocols. On the other hand, in third molar surgeries, photobiomodulation has been widely used in the treatment of postoperative complications such as pain, edema, and trismus. This study aims to evaluate the effects of photobiomodulation preconditioning in third molar surgeries. This is a clinical, randomized, double-blind, and placebo-controlled study. Adults over 18 years old, with an indication of removal of third molars, compatible with the inclusion criteria, after signing the Informed Consent Term, their facial and interincisal measurements were measured. They received treatment according to randomization (treatment group-TG / control group-CG), and after one hour, they underwent removal of the third molar. After 48h and 7 days, measurements were taken, pain analysis according to a visual analog scale and randomized treatment. At the conference, we will present the preliminary results of this clinical trial.

Biography:

Frederico Carlos Jana Neto is a physician (USP, Brazil). Medical residency in Orthopedics and Traumatology and Fellowship in Hip and Knee Replacements (IOT HCFMUSP). MBA in Health Management (Insper). Ph.D. student in Biophotonics and Professor at Nove de Julho University. Preceptor of medical residency in orthopedics and traumatology at Mandaqui Hospital Complex, SP, Brazil. Head of the Knee Arthroplasty Group at the Prevent Senior Institute.

Abstract:

Photobiomodulation (FBM) is indicated as an adjuvant treatment to accelerate wound healing, however, there is still a lack of evidence regarding its effect on traumatic soft tissue injuries. This project aims to evaluate the effects of FBM in the resolution of complex soft tissue injuries of traumatic origin associated with tibial fractures. 84 adult individuals, aged between 18 and 60 years, hospitalized with tibial fractures awaiting resolution of soft tissue injuries will be included to undergo definitive surgery. The subjects will be randomized in two groups: FBM (treated with a device with 144 LED emitting diodes at wavelengths of 420, 660 and 850nm, 3J per point for 10 minutes) and Sham (simulation of the LED application, with a device with characteristics identical to that of the FBM group, for the same period of time). Subjects will be treated daily until release for surgery. The primary outcome will be the assessment of the wound healing process using the Bates-Jensen scale. Secondary outcomes will be: pain intensity, consumption of analgesic drugs, serum evolution of inflammatory markers C-reactive protein and creatine kinase, measurement of the lesion area, the time needed for release for definitive surgery, presence of infection, and the effective effectiveness of FBM. The evaluations will be carried out before the beginning of the intervention and daily until the participant is considered ready for surgery (which will be considered the end of the experimental period). The data will be analyzed statistically, considering a significance level of 5%.

Biography:

Juliana Hironaka is a dentist with graduation and specialization in endodontics at Methodist University in Sao Paulo, Brazil. She received a degree of specialist in microscopy and endodontic microsurgery at the Association of Dental Surgeons of Sao Paulo in 2010.  With great interest in the field of biophotonics she started a research project at the University Nove de Julho relating endodontics and respiratory diseases in 2019. At the present time she works in the area of endodontics in a private clinic. Her future project involves teaching and researching in this field.

Abstract:

Apical periodontitis is caused by pulpal necrosis, with consequent inflammation and destruction of the periapical tissues. It has been shown that periodontal disease can influence the symptoms of Asthma. Asthma is a chronic inflammatory disease characterized by lower airway hyper responsibility and variable airflow limitation. To date, there are few papers correlating the symptoms of Asthma in patients with apical periodontitis (AP).This study aims to evaluate the effect of photodynamic therapy with endodontic treatment on pulmonary inflammation in an experimental model of Asthma. Forty-eight male Balb/c mice were divided into 6 groups (n = 8). Basal, AP, AP + PDT, Asthma, Asthma + AP, Asthma + AP + PDT. Apical periodontitis was induced with the coronary opening, and the tooth remained open for 21 days. Asthma was induced by ovalbumin (OVA) and aluminum hydroxide, subcutaneously (days 0 and 14) challenge via tracheal oro, three times a week for six weeks. The treatment of PA was performed with calcium hydroxide and PDT with methylene blue (0.005%) associated with red diode laser irradiated 660 nm, with the energy of 287 J / cm², with 9 J at the point, delivered in 90 s. Total and differential counts of alveolar bronchial lavage (BAL) and serum alkaline phosphatase were analyzed after euthanasia. We observed an increase of lymphocytes and eosinophils of BAL in the Asthma+AP group when compared to the Asthma group. On the other hand, we observed a reduction of this cell recovered from the BAL in the Asthma+AP+PDT group about the Asthma+AP group. In addition, there was an increase in bone resorption (alkaline phosphatase) in the AP and Asthma+AP groups, but with the treatments, only the non-asthma groups reduced this parameter. It is concluded that apical periodontitis may increase pulmonary inflammation. The association of Asthma with apical periodontitis worsens the parameters of pulmonary inflammation. However, after the standard treatment with PDT of apical periodontitis, pulmonary inflammation decreased in the asthmatic mice, reaching near the basal levels.

Biography:

Luciana graduated in Dentistry in the University of Santo Amaro (1999), a specialization in Periodontics from Paulista University (2004) and in Implantology in the Paulista Association of Dental Surgeons (2008). In 2019 she specialized in Training Teachers for Higher Education in the Nove de Julho University. She joined the master's degree in Biphotonic Applied to Health Sciences at the same institution, where she has a research project in the field of antimicrobial phototherapy in periodontics. She currently works as a specialist in periodontics and implantology in her private clinic and in the future, intends to teach, and continue researching in her area of expertise.

Abstract:

Periodontal disease (PD) is a chronic inflammatory disease caused by bacterial biofilm which is highly prevalent worldwide. Antimicrobial photodynamic therapy (aPDT) has been used as a complement to the conventional treatment and aims at the production of reactive oxygen species (ROS) using light and an exogenous photosensitizer to eliminate microorganisms. On the other hand, antimicrobial blue light therapy (aBLT) takes advantage of endogenous photosensitizers (such as protoporphyrin and flavin). The objective of the present study is to evaluate the potential for the bacterial kill by aBLT and the influence of the culture medium inthe death rate of Aggregatibacter actninomycetencomitans and Porphyromonas gingivalis. The protocol used will be the use of a 403nm ± 15 LED (blue / violet) with a power of 1W, the irradiance of 588, 2 mW / cm², for 0, 1, 5 and 10 min of irradiation exposure. Four groups are being studied: A. actinomycetencomitans cultivated in BHI; P. gingivalis grew in BHI; A. actinomycetencomitans grown in blood agar, and P. gingivalis grown in blood agar. The plates will be incubated in microaerophy and anaerobiosis, in a bacteriological greenhouse, with a temperature regulated at 37o C during a period of 48h to count the colony-forming units (CFU / mL) and performed in triplicate. The comet test will be carried out on the culture of microorganisms after irradiation to assess the possible DNA damage generated by antimicrobial therapy with the blue light. At the conference, we will present the first results of this work.

Biography:

Mara Lúcia Gonçalves Diogo Graduated in Nursing from the Nursing School WenceslauBraz (1986). Postgraduate in Occupational Nursing from the Catholic University of Santos (1989), the title of Specialist in Dermatology by the Brazilian Association of Nursing in Dermatology - SOBENDE (2007). He is currently a freelance professional, working in private practice. Experience in Nursing, with emphasis on Nursing in Dermatology and aesthetics. Invited professor in specialization courses in Dermatology throughout Brazil, done in person, or through online classes. Master's student in Biophotonics at Universidade Nove de Julho, São Paulo.

Abstract:

Light therapies have been used in dermatoses, including acne. Some studies indicate that the blue LED inhibits the proliferation of C. bacterium acnes and that the red light acts to decrease the inflammatory condition. Several studies have been analyzed, some in vitro, others in vivo; however, few studies have compared and analyzed the in vivo use of LED in acne. Therefore, the objective of this study is to compare the effect of the blue LED, the red LED, and the combined red and blue LEDs in patients with mild and moderate inflammatory acne (grades II and III). 108 participants with inflammatory acne will be selected according to the classification of the Brazilian Society of Dermatology and IGA (Investigator’s Global Severity Assessment) and also the degree of skin color according to the Fitzpatrick scale (I to IV). Participants will be treated with an LED mask, applied over the entire face, for 10 minutes, 3 times a week, in the UNINOVE laboratory, divided into 3 groups, where the first group will use a mask with blue LED, the second with LED red and the third with combined blue and red LED. The evaluations will be made through photos and counts of papules and pustules, in the baseline, on the 15th and 30th day of treatment by a blind evaluator. Participants will also assess their perception of improved appearance after the end of treatment, using the CADI questionnaire (CARDIFF ACNE DISABILITY INDEX).

Biography:

Paula Tatiane Alonso is currently pursuing a master's degree in the Post Graduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE-São Paulo-Brazil- since 2019). Physical Educator (UNICAMP- Campinas-Brasil- 2005) with Specialization in Physiology, Nutrition, Biochemistry, and Training (UNICAMP- Campinas-Brasil -2006), Specialization in Exercise Physiology (UNICAMP- Campinas-Brasil -2006), Specialization in Acupuncture (EBRAMEC-São Paulo-Brasil-2017) and Advanced Studies in Acupuncture and Moxibustion (TCM University of Shandong -China-2018). Currently teaches acupuncture photo course for students of Traditional Chinese Medicine. She is dedicated to the study of Photobiomodulation in respiratory diseases (especially asthma) and also pain and pathologies and syndromes in triathletes (and athletes in general).

Abstract:

Sepsis is a severe disease with high mortality index. Acute lung injury (ALI), characterized by alveolar damage, lung inflammation, and impaired gas exchange, is a serious manifestation of sepsis, which is not efficiently treated by current drugs. ALI is pathology with high morbidity and mortality. Photobiomodulation (PBM) has been showing good results for several inflammatory diseases. The aim of this study was to analyze the effect of systemic PBM with a red LED in the management of inflammatory parameters. Male rats were injected with lipopolysaccharide (LPS) or saline (i.p.) and, irradiated or not with light-emitting diode in the caudal vein, for 150 s, 2 and 6 h after the LPS injections. After 24 h the LPS or saline injections, the inflammatory parameters were investigated. Device specifications: Bio Lambda LEDstar, Black Box Mini Model, São Paulo, Brazil; Probe Design, Single Probe; Wavelength: 660 nm; Radiant Power: 160 mW; Power Density: 38,5 mW/cm²; spot area: 4,15 cm²; Density of energy: 5,8 J/cm²; Issuance: Continuous (cw); Total Radiant Emission: 24 J. Our results point to the beneficial effects of systemic PBM on the LPS-induced ALI, as it reduced the number of neutrophils recruited into BAL, myeloperoxidase activity and also reduced IL-1β, IL- 6 and IL-17 in the lung. Our data showed beneficial effects of transcutaneous systemic PBM treatment on ALI caused by sepsis, and suggest that PBM application as an inexpensive and non-invasive additional treatment to sepsis.

Biography:

Will be updated soon.

Abstract:

Chronic obstructive pulmonary disease (COPD) is characterized by chronic inflammation and alveolar enlargement. Several experimental models have been proposed for the discovery of new therapeutic options. Thus, mesenchymal stem cells (MSCs) and photobiomodulation (PBM) have been studied for their immune system modulating functions. The aim of this study was to investigate the effects of PBM associated with deciduous tooth MSCs on inflammatory mediators in bronchial epithelial cells (BEAS) induced by cigarette smoke extract (CSE). Human bronchial epithelial cells (BEAS-2B) were cultured (5x10⁴ cells / well) and after 24 hours, the cells were incubated with CSE. 1 hour later irradiated with 808nm, 30mW, 60 seconds / well diode laser and / or MSCs extracted from tooth pulp, cultivated and characterized by the presence of CD90⁺, CD73^+, and absence of CD34. After 24 hours, the supernatant was collected and the mediators were dosed. The effects of the association of PBM with MSCs on the release of cytokines (IL-6, IL-10 and IFN-g) were evaluated by ELISA.

Results: CSE increased levels of IFN-g (p<0.001), decreased levels of IL-10 (p<0.001), did not change levels of cytokines of IL-6. Groups treated with MSCs showed an increase compared to the other groups (p<0.001). There was a significant decrease in IFN-g (p<0.001), and an increase in the IL-10 (p <0.001) in the treated groups. In vitro treatment with MSCs and PBMs of CSE treated cells can be promising on the effects of the 808nm laser in the treatment of patients with COPD.

Biography:

Will be updated soon

Abstract:

Introduction: Since asthma is a multifactorial disease whose treatment sometimes is not effective, new therapies that improve the respiratory discomfort of patients are of great importance. Photobiomodulation (PBM) has emerged as an important toll to treat lung diseases characterized by inflammation. So our objective was to investigate the effects of PBM on allergic lung disease by an evaluation of lung cell migration, mucus secretion, tracheal responsiveness, and mast cell degranulation.

Methods: Male Balb/c mice were or not sensitized and challenged with ovalbumin (OVA) and treated or not with PBM (1h and 4 h after each OVA challenge). Twenty-four hours after the last OVA challenge the analysis were performed. Analysis was performed 24h the last LED treatment or OVA challenge. Device specifications: Bio Lambda LEDstar, Black Box Mini Model, São Paulo, Brazil; Probe Design, Single Probe; Wavelength: 660 nm; Radiant Power: 160 mW; Power Density: 38,5 mW/cm²; spot area: 4,15 cm²; Density of energy: 5,8 J/cm²; Issuance: Continuous (cw); Total Radiant Emission: 24 J.

Results: Our results showed that PBM treatment in asthmatic mice reduced the lung cell infiltration, the mucus production, the tracheal’s contractile response. The effects of PBM treatment on these parameters may be modulated by mast cells, since the degranulation was decreased (P<0.05).

Conclusions: This study may provide important information about the effects of PBM, and in addition, it may open the possibility of a new approach for the treatment of asthma.

Financial support: CNPq 305099/2017-5.

Biography:

Tainá Caroline dos Santos is a pharmacy and biochemistry graduate by the Universidade Nove de Julho (UNINOVE). She received her Master's Degree in Biophotonics Applied to the Health Sciences and Collective and Family Health specialization degree from Universidade Nove de Julho (UNINOVE). Currently, she is a Ph.D. student in Biophotonics Applied to the Health Sciences. Since her graduation, she was involved with the development of biochemistry educational tools. Her primary research interests are molecular biology and physiology. Specifically, she is interested in muscle injury and regeneration, inflammatory process, and photobiomodulation benefits. She is involved in studies with different experimental models.

Abstract:

Macrophages are key effector secreting products that can modulate the immune response during all phases of the inflammatory and tissue repair processes. Photobiomodulation therapy (PBM) with a low-level laser has been shown positive effects during the muscle repair process, modulating the cells involved in the inflammatory process, especially the macrophages. The aim of this study was to evaluate the effects of PBM on the synthesis of cytokines IL-6 and TNF-α in myoblasts cultivated in the presence of an M1 phenotype macrophage-conditioned medium previously submitted to the same irradiation parameters. J774 macrophages were activated with interferon-γ and lipopolysaccharide for 2h to induce the M1 phenotype. Irradiation was performed once using an AlGaAs diode laser (780 nm, 70 mW, 0.04 cm², 15 s, 1 J). C2C12 myoblasts were cultivated in a proliferation medium (DMEM + 10% FBS) and were irradiated with the same parameters used for the macrophages. After PBM, the myoblasts received 30% of M1 macrophage-conditioned medium (MCM1) from irradiated (+PBM) and non-irradiated macrophages. The IL-6 and TNF-α protein levels were detected 24 and 48h after C2C12 irradiation using ELISA kits. Untreated and PBM-treated myoblasts exhibited lower IL-6 levels in the presence of irradiated MCM1 at 24 and 48h. PBM treated myoblasts that received MCM1+PBM showed lower TNF-α levels after 24h in comparison to untreated myoblasts in non-irradiated MCM1. After 48h, untreated and PBM treated myoblasts exhibited lower TNF-α levels in the presence of MCM1+PBM. PBM performed concomitantly on myoblasts, and proinflammatory macrophages were able to modulate the synthesis of IL-6 and TNF-α protein levels.

Biography:

Cristiano Rodrigo de Alvarenga Nascimento graduated in medicine at the federal university of Juiz de Fora, the same college where he completed his medical residency in Pulmonology. Specialist in Pulmonology and intensive care and with an MBA from Fundação Instituto de Administração, Universidade de São Paulo. In 2016, he founded the Prevent Senior Institute for the eponymous institution, with a focus on research in the field of cell therapy, and created the medical residency of that institute, an important training program for specialist doctors. In 2018 Joined the doctoral program at Nove de Julho University, with a focus on biophotonics applied to health sciences. His main interests are photobiomodulation and stem cells.

Abstract:

Asthma is characterized by chronic inflammation of the airways, reversible airflow obstruction, and airway hyperresponsiveness. A common treatment is performed with the use of a corticosteroid such as Fluticasone (FT). Photobiomodulation (PBM) is relatively new, inexpensive, with no side effects, and demonstrates effectiveness in reducing inflammatory parameters. We aimed to evaluate the effects of PBM and its combination with FT in asthma. The Balb/C mice were divided into groups: controls, FT, PBM, OVA, OVA+PBM, OVA+FT, and OVA+PBM+FT. We induced inflammation by sensitization with ovalbumin - OVA and orotracheal challenge from day 21. We treated with FT (100 μg/kg - intranasal) one hour before challenge with OVA, and one hour after the challenge, we applied PBM - laser diode (660nm, 30mW, and 3J/cm²) at three distinct points. Twenty-four hours after the last treatment, the animals were anesthetized for the collection of bronchoalveolar lavage (BAL) and lungs. The data were submitted to the One-way ANOVA test, followed by the Newman-Keuls test. Significance levels adjusted to 5% (p<0.05). We observed a reduction in the total number of cells in BAL, in macrophages, with the best result in OVA+LLL, lymphocytes, with a greater reduction in the groups associated with the laser, neutrophils, and eosinophils. Reduction of production of cytokines IL-4, IL-5, IL-1β, TNF-α, and IL13, where the laser-treated groups showed better results, increased the level of the IL-10, presenting better results in the groups treated with FT. There was a reduction in collagen fiber deposition and mucus production in the airways.

Biography:

Rita de Cássia Ferreira is a graduate nurse from UFRJ (BRAZIL) and has a Master's degree in Biophotonics Applied to Health Sciences from the University Nove de Julho (Brazil). She is currently a PhD student at the UNINOVE graduate program (Brazil). Coordinator of the Dressing Group, member of the central wound prevention and treatment committee of the Government of the State of São Paulo (Brazil) and researcher at the Biophotonics Center of the Mandaqui Hospital Complex (São Paulo, Brazil). She works in research with an emphasis on photobiomodulation in chronic and acute wounds, pressure injuries and complex wounds.

Abstract:

Fournier's gangrene (FG) is necrotizing fasciitis of the perineum, abdominal wall, and genital regions of men and women. It is characterized by obliterating endarteritis with ischemia and thrombosis of the subcutaneous vessels with necrosis and bacterial infection. 1,2 Surgical interventions accompanied by long periods of hospitalization along with coverings and dressings are the Treatment of choice for FG, making Treatment long and costly for the public health system. This study aims to present a case report on a patient with FG who underwent photobiomodulation (PBM) associated with secondary covering. Description: WOM, male, 49 years old, admitted to a public hospital in the state of São Paulo on 15 Oct. 2019 with a diagnosis of FG; emergency surgical debridement was performed accompanied by antibiotic therapy. The PBM was applied to wound with a cluster: 3 LEDs 460nm, P= 400Mw and 2 lasers 600nm, P= 100mW and 2 red LED emitters, 660nm P = 200mW; 60 seconds per points

Secondary dressing with FITOSCAR® (extract of Stryphnodendron adstringens) was changed every 48 hours for the ten days of Treatment. The lesions were evaluated using the PUSH scale with the improvement of the border, secretion, and wound center. It was concluded that PBM associated with FITOSCAR® was satisfactory in the Treatment of FG, reducing hospitalization time as well as hospital costs.