Jinan University, China
Optical fiber based nano manipulation
Will be updated.
With the observation of small objects, precise manipulation is also highly desirable, especially for three-dimensional manipulation of nanoparticles or biomolecules with a size of less than 100 nm . Although optical tweezers have become powerful tools to manipulate microparticles and cells, they have limits when extended to the nanoscale because of the fundamental diffraction limit of light. The emergence of near-field methods, such as plasmonic tweezers and photonic crystal resonators, has enabled surpassing the diffraction limit. However, these methods are usually used for two-dimensional manipulation and may lead to local heating effects that will damage the biological specimens. Therefore, we propose a near-field technique that uses a photonic nano jet to perform the three-dimensional optical manipulation of sub-100-nm objects. With the photonic nano jet generated by a dielectric microlens bound to an optical fiber probe, three-dimensional manipulations were achieved for fluorescent nanoparticles as well as for plasmid DNA molecules . Backscattering and fluorescent signals from the trapped targets were detected in real-time with a strong enhancement. The demonstrated approach provides a potentially powerful tool for nanostructure assembly, biosensing, and single-biomolecule studies.
Fig. 1. Schematic illustrating the manipulation of a nanoparticle by a photonic nano jet
Behnaz Abbasgholi Nejad Asbaghi
Chemistry and Chemical Engineering Research Center of Iran (CCERCI), Iran
Thermal Lens Detection of DNA Using Unmodified Gold Nanoparticles in Microchip
Behnaz Abbasgholi Nejad Asbaghi is studying analytical chemistry at the chemistry and chemical engineering research center of Iran (CCERCI), Iran. She graduated as MS in 2012 at the same research center. Her Ph.D. thesis is supervised by Dr. Shokoufi at the Spectroscopy and Instrumentation Laboratory. This thesis focuses on the development of the photothermal lens technique for analysis of biomolecules and the investigation of DNA hybridization using gold nanoparticles in a microchip. Recently, she has published two papers about the analysis of biomolecules by photothermal lens spectroscopy.
In this work we report on a new sensitive method for detecting DNA by photothermal lens microscopy in a microfluidic chip. A specific sequence of DNA was detected using unmodified gold nanoparticles in a glass microchip withY-shaped channel. The different adsorption affinity of single and double-stranded DNAs on gold nanoparticles was used for highly rapid and sensitive DNA detection by photothermal lens effect in a femtoliter scale of detection volume.
Under the optimized conditions, the focal volume of 105 fL (10-15 L) was obtained as detection volume. The variation of photothermal lens signal in the detection volume was linearly proportional to the target DNA concentration over the range of 50-500 nM with a detection limit of 29 nM for target DNAs. The lowest amount of target DNA that was measured in the detection volume was 2.6 zepto mole. The assay was completed within 5 min, and the relative standard deviations (n=8) for both target DNAs were about 2.34%.
Two different common methods, including gel electrophoresis and in situ fluorescence monitoring of DNA hybridization, were used for proving the hybridization in this method. The proposed detection method was successfully performed in diluted human serum samples with recovery values between 98 % and 104.9%. The method is fast and homogeneous because it occurs exclusively in the liquid phase, a feature that makes it easy to be applied for online monitoring in the flow mode and lab-on-chip applications. This presentation encourages the researchers to develop this method for recognition of single-base-pair mismatches between probe and target, which is highly important in the biological detection of single nucleotide polymorphisms.
Georgian Technical University, Georgia
Monitoring and Diagnosis of the Process of Shrinkage and Crack Formation in Concrete Using Holographic Interferometry
Giorgi Dalakishvili joins Georgian Technical University (GTU) as a Professor in the Department of Hydro Engineering in 2010. Prior to attending GTU, he was a Head of Department of Management, material-technical and social base for the development at Ministry of Education and Science of Georgia. Up to 1996, he was Head of Department at Institute of Structural Mechanics and Earthquake Engineering. He received her MBA from Georgian Technical University and a Ph.D. from the Institute of Structural Mechanics and Earthquake Engineering of the Georgian Academy of Sciences. He has developed new courses as a Professor at GTU. His primary research interests are in the field of Holographic Interferometry and its applications in monitoring and diagnosis of the process of shrinkage and crack formation in concrete. He was a scientific head of some magister and doctoral thesis. Specifically, he is interested in student experimental works, as well as pedagogies. In his free time, he enjoys traveling.
Concrete is the main building material for hydro construction. Therefore, it is very important to study long-term processes, such as shrinkage, creep, swelling, temperature, and crack resistance, which affects its strength. Known methods do not fully describe these processes in materials such as concrete and reinforced concrete. In this paper, an application of holographic interferometry is considered which enables to evaluate qualitatively and quantitatively the deformation of a solid body and the processes of shrinkage, crack formation and development of concretes of different compositions. A holographic plate is exposed twice before chemical processing - for the first time when the surface of the test sample is in the initial condition, and the second time when it is deformed or undergoes any other impact. This is a unique way to simultaneously observe a unified picture of deformation on the whole registered surface of the object understudying and, at the same time, to measure all three components of the displacement vector at any chosen point. Experimental studies are discussed, in particular, such long-term processes as shrinkage and cracking for various fillers and various reinforcing materials. The improvement and development of research methods, as well as a broad introduction of its results in practice, which will further facilitate the reliability and durability of concrete and reinforced concrete structures, one of the major building materials in construction, in particular in hydro-technical construction. This will allows for monitoring and diagnostics during dam construction.
FZI Research Center for Information Technology, Germany
Capabilities and Limitations of a New Thermal Finite Volume Model for the Evaluation of Laser-Induced Thermo-Mechanical Retinal Damage
Markus Lücking studied engineering at the Technical University of Braunschweig and Zaragoza. He´s working as a research scientist at the FZI Research Center for Information Technology. His research interest is in the modeling of laser-tissue interactions.
Many experimental studies have been focusing on physical damage mechanisms of short-term exposure to laser radiation. In the nanosecond (ns) pulse range, damage in the Retinal Pigment Epithelium (RPE) is most likely to occur at threshold levels due to bubble formation at the surface of the strongly absorbing melanosome. In order to model the bubble formation threshold and, therefore, the damage threshold, the energy uptake of the melanosomes is one key aspect. In this work, a thermal finite volume model for investigations of temperature rise and temperature distribution of irradiated melanosomes is presented. The model takes different geometries of the melanosome into account as well as heterogeneous energy absorption of the melanosome. For the first time, the effect of size and shape variation on the melanosomes' thermal behavior is considered. The calculations illustrate the effect of the geometry on the maximum surface temperature of the irradiated melanosome and thus the impact on the bubble formation threshold. A comparison between the calculated bubble formation thresholds and the RPE cell damage thresholds within a pulse range of 3 to 5000 ns leads to a mean deviation of with a standard deviation of . The best agreement between the simulation and available RPE cell damage thresholds is achieved for pulse durations close to the thermal confinement time of single melanosomes.
Research Institute of Virology, Uzbekistan
Application of Unusual Properties of Low-Level Laser Radiation for Transfer Information from Medicine to Patient's Body in Therapy of Patients with Some Viral Diseases
Naylya Djumaeva has completed her Ph.D. at the age 40 years from Institute for Epidemiology, Microbiology and Infectious Diseases (Uzbekistan) Naylya works as the Consultant – Neurologist at the Research Institute of Virology(Uzbekistan) She is the author of 36 papers including reputed journals, and she has 2 patents.
This poster presents the results of the study of torsion field application in medicine Torsion fields were used with the aim to transfer information from the medicine to the patient's body. The group of patients with different chronic conditions such as chronic hepatitis B and C virus infections, patients with cytomegalovirus infection, patient with Epstein- Barra infection was treated with the application of laser light guide, where the source of light was low-level laser radiation. The patients were under observation in the Out-Patients Department of Research Institute (Uzbekistan), and the approach to therapy towards the patients was the same: torsion fields formed by the radiation of low-level laser therapy device. Into the field of radiation, the medicine was placed to be transmitted to the patient's body. Our approach to therapy has patented. The obtained results have shown that under the torsion field, formed with the help of a laser light guide, the distant transmission information from medicine to the human body occurred. The method of therapy is safe and might be used to treat patients with chronic viral diseases. In some cases, it results in the complete elimination of the virus infection (hepatitis C virus infection). In patients with a virus pathology of the liver, this therapy leads to the improvement of quality of their life and contributes to the prevention of the development of such terrible complications of chronic viral such as cirrhosis of liver and cancer of the liver