Biography:

Abstract:

Presenter Local Time: 11:55 AM-12:15 PM
The electromagnetic field interaction with a multilayered structure having a periodic interface and comprising slabs of smart artificial materials (e.g. metamaterial or graphene) is studied by means of the modified C-method furnished with certain elements of the analytical regularization technique.

To understand the process of interaction of electromagnetic radiation with complex structures, like the one depicted in Fig. 1, it is necessary to have a full picture of the behavior of its constitutive elements in the electromagnetic filed.

A special attention should be payed to the study of a dielectric slab with periodic interface. Such substrate is widely used for installing and growing graphene or metamaterials layers. It is responsible for protection and tuning, distortion of undesirable resonances, for hiding or for blinding [1]. Introduction of periodicity, which is a strongly transformative interface for electromagnetic waves, provides engineers with additional tools for electromagnetic control of coating sandwiches [2].

The process of interaction of electromagnetic waves with periodic interface of dielectric slab (backed with metamaterials or graphene monolayer in further applications) is modeled by means of a boundary value problem. It is resolved using the C-method with regularization [3], [4]. This approach ensures reliable results in numerical simulations. The predictable and set-in-advance accuracy of calculations is especially important for the study of super high-Q resonances, which are typical to the structures of interest.

Extensive research on the control of resonances by optimizing the geometry of the structure and changing their electromagnetic properties has been carried out.

The obtained results could serve as a basis for applications of smart coatings in various wavelength ranges and geometrical scales.

Biography:

Patricia completed her Bachelor’s degree in dentistry from Unifenas University. She persued her Post Graduate’s degree "lato sensu" specialist level in Orthodontics, Functional Jaw Orthopedics. Qualified laser therapiest from USP. 
Her specialization includes Orofacial Harmonization, Temporomandibular JointDisorder and Orofacial Pain and Biophotonics.

Abstract:

Presenter Local Time: 7:10 AM-7:25 AM
Rheumatoid arthritis (RA) is classified as a chronic inflammatory disease of the joints,due its systemic autoimmune alterations, which lead to progressive irreversibledegenerations of the cartilage and bones involved in the joints. RA affects about 0.5%to 1% of the global population, predominantly women. Experimental studies "in vivo"have shown that through photobiomodulation (PBM) therapy, the inflammatorymodulation has been shown to be positive. The purpose of this study is to verify an "invivo" sample in order to evaluate the effects of PBM therapy on the control of theinflammatory process and pain in an experimental model of RA. In the methodologyof this work, it will be used male Wistar rats that will be divided into 4 groups: CTL(control), RA NT (Rheumatoid Arthritis not treated), RA LP (Rheumatoid Arthritis treatedwith local photobium), and RA VP (Rheumatoid Arthritis treated with vascularphotobium systemic effect). For the induction of RA, the parameters of a previous studywill be used, where the animals received 2 intradermal + systemic injections of thelesion-inducing solution on days 0, 7 and 21. For the groups to be treated, Local PBM(808nm; 6J; 100mW) and Vascular PBM (808nm; 180J; 100mW), will be introduced afterthe last induction. It will be noticeable the histological analysis of tissue organization andinflammatory infiltrate; Functional tests (Allodynia and mechanical properties);Biochemical/Molecular analysis (RT-PCR, Inflammatory mediators and Receptorsinvolved in pain). The statistics that will be used: Means of the MPD and ANOVA (oneway)with Tukey's post hoc.

Biography:

Prof. Zhengang Lu is Professor of School of Instrumentation Science and Engineering at Harbin Institute of Technology (HIT). He obtained the Bachelor, MD and PhD in Instrument Science and Technology from HIT in 2001, 2004 and 2007 respectively. He is the associate director of Key Laboratory of Ultra-precision Intelligent Instrumentation (HIT), Ministry of Industry and Information Technology, and head of the research group of micro-nano optics and electromagnetic shielding optical windows. He is a member of Optical Testing Committee of Chinese Optical Society, executive director of Mechanical Testing Instrument Branch of China Instrument and Control Society, a member of the National Technical Committee for Standardization of Electromagnetic Shielding Materials (SAC / TC323) and Micro Electro Mechanical Technology (SAC /TC336). He is also young communication expert of Engineering and ?FITEE?of Chinese Academy of Engineering. His research interest is mainly focus on micro-nano optics and electromagnetic shielding optical windows, precision instruments and engineering. He authorized 41 invention patents, and published more than 30 academic papers in Advanced Science Engineering, Nanoscale,ACS Applied Materials & interfaces, Applied Physics Letters, Optics Letters, Optics Express and other journals.

Abstract:

Presenter Local Time: 5:15 PM-5:35 PM
Cracks and increase in sheet resistance harm the electromagnetic shielding effectiveness (SE) of metallic mesh films. It is necessary to find a easy way to detect these defects. By modeling the metallic mesh as a two-port network, the relationship between the transmittance and complex reflection coefficient was established to evaluate SE through vector reflection measurement. By introducing the time domain gating technique to eliminate interference from the multipath effect, a test deviation of no more than 0.95 dB @ Ku-band compared to the standard SE measurement was observed in a non-anechoic environment, which demonstrates the validity of the proposed method. Moreover, a complementary spiral resonator (CSR) operating at 7.05 GHz is developed to distinguish two typical defects by a downshift in resonant frequency and decrease in quality factor respectively. The CSR was then integrated into a feedback oscillator with an additional loop filter to achieve self-sustained measurement and self-switching effect for crack. The experimental results show that the developed sensor can detect cracks with 0.4 mm width and a 3.6 % increase in sheet resistance.

Biography:

Dr. Jose Maria Aguilera is a Medical Director in Centro Láser Médico Quirúrgico Corpsnatura and  Thermo Health Diagnostic and Reseach Center. Asunción, Paraguay.

He is specialized in Phlebology and Lymphology and an internacional medical laser specialist.

MEMBER OF THE EDITORIAL BOARD OF LASER THERAPY MAGAZINE

Published by International Academy for Laser Medicine and Surgery, International Phototherapy Association, Asian Pacific Association for Laser Medicine and Surgery, International Society for Laser Surgery and Medicine Japan Association for Laser Medicine and Sports Science, World Federation of Society for Laser Medicine and Surgery.

Abstract:

Presenter Time:9:30 AM-9:50 AM
Biopolymers are used commonly as fillers for aesthetic treatment in different areas, most often used in Face, Breast and Gluteal Region. Adverse effects are noticed like Edema, Stiffness of the area, pain, Granulomas, change of skin color. With the stablish of ASIA syndrome, and other clinical criteria to determine body compromise due to this reaction to the fillers. The variety of reactions, signs and symptoms are very large, and certainly unknow for the general physician.

The Objectives of this pilot study are the Assessment, Clinical Treatment, and Minimally Invasive Laser Surgery for the extraction of the major portion of foreign body reaction due to the biofillers on the human body.

In this pilot study, horizontal and prospective, with follow up post-surgical procedure are confirmed by three cases of foreign body reaction with ASIA Syndrome criteria. The biophotonic assessment done by Infrared Medical Thermography, and once the clinical criteria are concluded, the planning for the complete assessment, clinical management, laser surgical plan and procedure are designed for every case in particular.

Biography:

Matt Kalinski (born 1968) is US theoretical physicist who discovered Trojan wave packets, sqeezed, coherent and intrinsically coordinate-entangled states of electrons in true atoms solving the long standing problem of interstellar rocket propulsion by extending the positron or positronium lifetime and control the arbitrary slowdown of the recombination process of antimatter in positronic rocket engine. Kalinski earned his PhD in Physics from the University of Rochester. The broad applications of his discovery of coherent non-dispersing electrons and electron pairs in atoms and polar molecules are important and not limited to photonic superconductivity, laser centrifugal isotope separation of Deuterium, theory of cold nuclear fusion in Palladium, detection of ultra-weak magnetic fields with Aharonov-Bohm effect, direct observation of Berry phase in single atoms, arbitrary quantum state preparation, observation of Unruh-Davies effect as well as for the detection of possible gravito-electromagnetic force and twisted corrections to Einstein equations and precise engineering of complex quantum dot systems. 

Abstract:

Presenter Time: 7:10 AM-7:30 AM
Some time ago we have discovered that placing the Langmuir trajectories [1] of the type one i.e those in what we called the ”Hoop Earrings” configuration in a combination of the symmetry augmented Circularly Polarized (C.P.) electromagnetic field and the magnetic field perpendicular to the planes of the both electron parallel circular motions results in classical stabilization of the resulting Langmuir trajectories which therefore can support the stable non-dispersing quantum Trojan Wave Packets.

We have also recently shown that the Langmuir trajectories of the type two i.e. those corresponding to the popular toy, the Click-Clack Balls when two electrons are moving in one plane on the semi-circular trajectories with the opposite angular velocity, they bounce from each other, reverse the velocities and continue bouncing again and again also support such packets. To stabilize and confine the system further the perpendicular static magnetic field can be added in addition to the resonant L.P. field.

We have also found the nondispersing wavepackets in the joined combination of the external L.P. field and the parallel static magnetic for the several electrons which planes of the semi-circular bouncing motion are symmetrically oriented with respect to each other under the multiples of the integer fraction of the full 360 degrees angle.
We use the generalized Gaussian ansatz

for the packet wave function and solve the equations for the localization matrix M(t) together with the classical equations of the motion.

Biography:

Haobin Zheng received the B.S. and M.S. degrees from National University of Defense Technology, Changsha, Hunan province, China. Now, he is a technician in the Department of Physics, College of Science, National University of Defense Technology, Changsha, Hunan province, China. His research interests include fiber lasers, nonlinear optics, and spatial filtering.

Abstract:

Presenter Local Time: 4:00 PM-4:10 PM
The state-of-the-art mode-locked erbium-doped fiber lasers with a non-linear amplifying loop mirror (NALM) still have a low repetition rate. For the non-polarization-maintaining scheme, the highest is 257 MHz [1]. For the polarization-maintaining scheme, the highest is 250 MHz [2]. For the mode-locked fiber laser with a NALM, the self-starting of mode-locking is closely related to the differential non-linear phase shift (NPS). Thus, the cross-phase modulation (XPM) effect definitely makes a contribution to pulse formation and self-starting in the mode-locked fiber lasers, which has not been thoroughly studied yet.

In order to overcome the difficulties of increasing the repetition rate and analyze the influence of XPM on self-starting of mode-locking, we calculate the power distributions of two counterpropagating beams in the NALM and the differential NPS accumulations. Our analysis is carried out from the perspective of NPS accumulation. We find a difference between the differential NPSs for the CW light and the pulses in the fiber loop, which makes the NALM show an inverse saturable absorption (ISA) mechanism during the pulse formation. The ISA has been extensively studied in the real saturable absorber [3], but not in the artificial saturable absorber. The ISA in the NALM could be used to explain the experimental phenomena that the mode-locking of laser can be actively started by tapping fiber, fine-tuning light polarization, or other disturbances. These results are helpful for optimizing the design of NALM and lowering the self-starting threshold of the high-repetition-rate mode-locked fiber laser. 

Biography:

I am currently pursuing a master’s degree in Biophotonics applied to Health Sciences at UNINOVE, supported by a CAPES scholarship. In 2023, I completed a specialization in the treatment of Special Needs Patients at the Hospital das Clínicas, Faculdade de Medicina/Universidade de Sao Paulo. Additionally, I have expertise in Family Health, acquired through the Multi professional Residency Program in Family Health in São Bernardo do Campo in 2021. Furthermore, I underwent specialized training in digital dentistry with laser certification at UNINOVE in the same year. I earned my undergraduate degree in Dentistry from the Universidade Nove de Julho/ UNINOVE in 2017, during which I actively participated in the Scientific Initiation Project centered on Applied Biophotonics to Health Sciences, with a specific focus on Cell Culture, Immunology, and Molecular Biology.

Abstract:

Presenter Local Time: 8:45 AM-8:55 AM
The connective elements within skeletal muscle perform structural roles and are pivotal in the process of muscle regeneration. They contribute to the modulation of inflammation and the regeneration process, a critical aspect of recovery from muscle injuries. Such injuries can result in functional limitations and discomfort for individuals, including both high-performance athletes and sedentary adults. The organization of connective tissue post-injury plays a vital role in maintaining optimal muscle function. Extensive literature supports that Photobiomodulation (PBM) can effectively modulate inflammation, reduce myonecrosis, and enhance the diameter and cross-sectional area of muscle fibers. Therefore, the aim of this study was to investigate the impact of transcutaneous vascular PBM (VPBM) administered prior to inducing acute muscle injuries in rats, on the overall collagen deposition within the healing muscle tissue following cryoinjury. A total of 15 Wistar rats were used and divided into the following experimental groups: (1) Control; (2) Injury; (3) Previous VPBM + Injury Group. The animals were submitted to the cryoinjury on the anterior tibial muscles (TA). VPBM administration was performed once, 24h before cryoinjury procedure, by irradiating the tail artery/vein (AlGaAs, 780 nm, 40 mW, 10 J/cm², 3.2 J). Euthanasia was performed on day 2 after inducing the injuries. Muscle samples were collected, processed histologically, and stained with Picrosirius Red (Sigma-Aldrich, St. Louis, MO, USA) to quantify the total collagen area using ImageJ software. The results were subjected to statistical analysis (ANOVA/Tukey). The findings revealed a consistent organization and distribution of collagen. Notably, the Injury group exhibited a significant increase in collagen compared to the Control group, whereas the Previous VPBM + Injury group showed a significant reduction in collagen. In conclusion, prior VPBM effectively modulated collagen deposition in skeletal muscle tissue during the repair process following acute injury However, additional studies are required to delve further into the mechanisms of VPBM, especially concerning the relation to optimal dosimetric parameters.

Biography:

Roberta Cruz Bueno,Graduated in Biomedical and Forensic Sciences and qualified in aesthetics and clinical analysis.Postgraduate in Aesthetic Biomedicine and Biomedical Engineering.Technical consultant in ablative and non-ablative fractional high-power lasers IPL, Cryolipolysis, Plasma Jet and Microfocused Ultrasound.Speaker from the company Celebrim Importações. Speaker from the company VICAE Cosmetic. Postgraduate Professor in Aesthetics at Faculdade IPESSP Lecturer at Instituto Diogo Lustosa Orofacial Harmonization and Facial AestheticsProfessor of Graduate Studies in Aesthetics at USCS. Master's student in Biophotonics in Sciences applied to health.

Abstract:

Presenter Local Time: 7:55 AM-8:05 AM
Skin aging is characterized by a progressive loss of functionality and regenerative potential. With increasing life expectancy, people are becoming more concerned about their appearance, increasing the demand for facial treatments. For nearly 15 years the fractional CO2 laser was considered the gold standard for facial treatment, however it is no longer being widely used due to the long skin recovery period and the risk of serious side effects. Aesthetics professionals have used photobiomodulation (PBM) to increase circulation and lymphatic drainage and mainly because of its anti-inflammatory effects after procedures with the potential to generate inflammation. However, most procedures that generate inflammation are also collagen inducers. Thus, it is plausible to admit that if the inflammatory process is reduced with the use of PBM, collagen production may be reduced and thus the result may be impaired. Thus, therapeutic resources that minimize the side effects of fractional CO2 laser are relevant. Therefore, the objective of this study will be to evaluate the role of PBM in the colagen production after the fractional CO2 in the skin. For this purpose, male Wistar rats will be submitted to CO2 laser injury on the skin and treated or not with PBM. After 21 days, the collagen production as well as mechanical properties of skin will be evaluated. This study will bring scientific evidence on the association of PBM with the CO2 laser.

Biography:

Abstract:

Presenter Local Time: 7:35 AM-7:45 AM
The multifocality and unpredictability   of actinic keratosis lesions with the potential progression to squamous cell carcinomas, and metastasis risk, make the selection of a therapeutic regimen a challenge. Additionally, the increasing incidence, associated economic costs, and impact on quality of life have leads the investigation of new protocols for the treatment of this serious skin condition. For these reasons, the aim of this study is to compare the efficacy of topical application of 8% and 16% methyl aminolevulinate (MAL) followed by red light irradiation in two therapeutic regimens of 1 hour and 3 hours in the treatment of facial actinic keratoses with a 3- month follow-up. One hundred and sixty participants will be randomized into 4 groups: G1 (control) MAL 16% with 3 hours of incubation, G2 (experimental) MAL 16% with 1 hour of incubation, G3 MAL 8% with 3 hours of incubation, and G4 MAL 8% with 1 hour of incubation. All irradiations will be performed with LED 630nm, 160mW/cm2, and 300W of power. The primary outcome will be the clinical resolution of the lesion in relation to the number of lesions within a 30-day period. Secondary outcomes will include the overall improvement of photo- damaged skin with actinic keratosis, side effects such as pain, erythema, edema, and desquamation after treatment and at 7 and 30 days, and the recurrence rate of treated actinic keratoses during the 3-month follow-up period. We will use the Kruskal-Wallis test for non- parametric data and ANOVA for parametric data. The P value will be set at 0.05.

Biography:

Aline Souza Silva is a Physiotherapist who graduated from Universidade Nove de Julho/UNINOVE in 2021. She initiated her research career during her undergraduate studies in the Biophotonics program and she is currently a Master's degree student holding a scholarship in the Rehabilitation Sciences program at the same university, recognized for her excellence. She is also currently a postgraduate student in Exercise Physiology Applied to Clinical Practice at the Universidade Federal de São Paulo/UNIFESP.

Abstract:

Presenter Local Time: 8:15 AM-8:25 AM
Fibroblasts are stromal cells responsible for the production and remodeling of the extracellular matrix and play a central role in the wound healing process. Compound diverse tissues, these cells are responsible for contractions that contribute to wound closure, with such function occurring depending on their phenotypic differentiation. Recently published studies using photobiomodulation (PBM) with low-level laser or LED showed an improvement in proliferation, differentiation and an influence at mitochondrial and reticular activity. However, a comprehensive understanding of the ideal dosimetric parameters of PBM to stimulate wound regeneration has not yet been achieved. Therefore, this study aims to assess different dosimetric parameters of blue LED light on the L929 fibroblast cell viability. The fibroblast L929 cells line (mouse cells) was cultured in a proliferation medium composed of Dulbecco’s modified Eagle medium (DMEM, Vitrocell, Campinas, SP, Brazil) supplemented with 10% fetal bovine serum (FBS, Vitrocell, Campinas, SP, Brazil) and 1% antibiotic–antimycotic solution incubated (HEPA class 3110, Thermo Electron Corporation, OH, USA) at 37 °C in a humidified atmosphere with 5% CO₂. The cells were removed from the culture flask and centrifugated. The L929 cells were divided into the following experimental groups: (1) Control, (2) PBM 4 J, (3) PBM 6 J and (4) PBM 8J. The PBM treatment was performed using blue LED (Quantum, Ecco ^®, 470 nm, 400 mW, 10 s, 15 s and 20 s and total energy of 4, 6 and 8 J, respectively) at the bottom of conic (Falcon) tubes. The cells were plated (2 x 10⁴) in 96-well culture plates and submitted to MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay for evaluation of cell viability after 24 and 48h of incubation. The results showed that after 48 h there was an increase in cell viability in the 8 J group in comparison to the 6 J group. However, after 24 hours, there was no statistically significant difference observed among the experimental groups. In conclusion, the application of blue LED light with a total energy of 8J demonstrated the capability to stimulate time-dependent L929 cell viability.   

Biography:

Abstract:

Presenter Local Time: 11:55 AM-12:05 PM
We discussed the creation of entanglement between a three level atom in a Ladder type configuration and itsspontaneous emission. The entanglement is affected by parameters such as coherent driving fields’ intensities, their relative phaseand incoherent pumping field’s rate. We also discussed the entanglement evolution due to the spontaneously generated coherence(SGC).
I-INTRODUCTION
One of the most dedicated properties of quantum mechanics which differentiates it from classical physics isentanglement. In quantum entanglement, there are states of composite systems (pair of particles say) that cannot be"factorized'' into separate states for the component subsystems so that any measurement on one subsystem may effecton the other, this has made entanglement as a potential tool in a variety of new technologies.
III. EQUATIONS, RESULTS AND DISCUSSION
We consider a Ladder type atomic system as it is shown in figure.1, and numerically calculate the entanglement ofthis system and its spontaneous emission fields via quantum entropy measurement.


The basic density equations of motion for spontaneous emission which is obtained from the general master equationof a multilevel atom in an arbitrary configuration of the energy levels takes the following form for a Ladder configuration,

Biography:

Haobin Zheng received the B.S. and M.S. degrees from National University of Defense Technology, Changsha, Hunan province, China.Now, he is a technician in the Department of Physics, College of Science, National University of Defense Technology, Changsha, Hunan province, China. His research interests include fiber lasers, nonlinear optics, and spatial filtering.

Abstract:

Presenter Local Time: 4:00 PM-4:10 PM
The state-of-the-art mode-locked erbium-doped fiber lasers with a non-linear amplifying loop mirror (NALM) still have a low repetition rate. For the non-polarization-maintaining scheme, the highest is 257 MHz [1]. For the polarization-maintaining scheme, the highest is 250 MHz [2]. For the mode-locked fiber laser with a NALM, the self-starting of mode-locking is closely related to the differential non-linear phase shift (NPS). Thus, the cross-phase modulation (XPM) effect definitely makes a contribution to pulse formation and self-starting in the mode-locked fiber lasers, which has not been thoroughly studied yet.In order to overcome the difficulties of increasing the repetition rate and analyze the influence of XPM on self-starting of mode-locking, we calculate the power distributions of two counterpropagating beams in the NALM and the differential NPS accumulations. Our analysis is carried out from the perspective of NPS accumulation. We find a difference between the differential NPSs for the CW light and the pulses in the fiber loop, which makes the NALM show an inverse saturable absorption (ISA) mechanism during the pulse formation. The ISA has been extensively studied in the real saturable absorber [3], but not in the artificial saturable absorber. The ISA in the NALM could be used to explain the experimental phenomena that the mode-locking of laser can be actively started by tapping fiber, fine-tuning light polarization, or other disturbances. These results are helpful for optimizing the design of NALM and lowering the self-starting threshold of the high-repetition-rate mode-locked fiber laser.Biography

Biography:

Aline Figueiredo Salu Catoia, graduated in nursing from Nove de Julho University and specialization in progress in dermatological and aesthetic nursing from the University of Midwest Paulista. She is a student of the Master's Program in Biophotonics applied to Health Sciences at Nove de Julho University. At the moment, she works at a dermatological clinic in São Paulo where she is the nurse responsible for high-power laser protocols, she also works with treatments in the gynecological area and slimming with serum therapy and injectables.

Abstract:

Presenter Local Time: 7:45 AM-7:55 AM
Polycystic Ovary Syndrome (POS) is a complex gynecological condition often associated with infertility and various metabolic risks, including the development of type II diabetes mellitus, dyslipidemia, cardiovascular disease, hypertension and metabolic syndrome. Several theories may explain the pathophysiological mechanisms involved in POS, which include: neuroendocrine defects, androgen synthesis/metabolism and/or action, cortisol metabolism, insulin action and/or secretion, and defects in lipid metabolism, as well as chronic subclinical inflammatory processes Conventional treatment involves drugs including metformin, orlistat and statins, but the long-term benefits are not completely clear; in addition they induce some side effects. In this sense, photobiomodulation (PBM) emerges as a promising therapy since it exerts important anti-inflammatory effects. Thus, the aim of this study will be to evaluate the effects of photobiomodulation on ovarian function in an experimental model of polycystic ovary syndrome. For this purpose, female Wistar rats will be used, submitted or not to POS induction by intramuscular administration of a single dose of estradiol valerate (2mg/0,2 ml) and treated or not with PBM (810 nm 100mW, 150s) in two points: right side and left side of the ventral surface. We will investigate clinical parameters such as: body weight, ovarian weight, quantification and gene expression of cytokines in ovarian homogenate, analysis of sex hormones, glucose and cholesterol. Considering that POS is a multifactorial disease that can induce infertility, the search for new treatments without side effects and at a lower cost is relevant.

Biography:

Dr. Erika is a Dental Surgeon graduated in 2006 from Umesp. Specialist in Orthodontics and Facial Orthopedics, Orofacial Harmonization. Master's student in Medicine with emphasis on Biophotonics.

She has completed International Certification in Facial Harmonization  from Harvard University and International Certification in Facial Harmonization from New York University. Professor of the postgraduate course in Orofacial Harmonization at INRO.

A columnist and consultant for the Dental Cremer Blog. More than 2000 hours in improvement courses in orofacial harmonization.

Abstract:

Presenter Time: 7:00 AM-7:10 AM
Skin aging is characterized by a progressive loss of functionality and regenerative potential. There is also a reduction in hyaluronic acid and glucosaminoglycans, as well as a decrease and disorganization of collagen and elastic fibers, a reduction in vascularization, hydration and lipid content. The concern with delaying aging associated with the increase in life expectancy makes people seek more and more facial treatments. For nearly 15 years the fractional CO₂ laser was considered the gold standard for facial treatment, however it is no longer being widely used due to the long skin recovery period and the risk of serious side effects. Thus, therapeutic resources that minimize the side effects of fractional CO₂ laser are relevant. Aesthetics professionals have used photobiomodulation (PBM) to increase circulation and lymphatic drainage and mainly because of its anti-inflammatory effects after procedures with the potential to generate inflammation and its signs such as edema, redness and pain. Therefore, the objective of this study will be to evaluate the role of PBM in the inflammatory process generated after the fractional CO₂ and the resolution process in the skin. For this purpose, male Wistar rats will be submitted to CO₂ laser injury on the skin and treated or not with PBM. After 7 days, the inflammation will be evaluated through the release and gene expression of inflammatory and resolution mediators in the skin. This study will bring scientific evidence on the association of PBM with the CO₂ laser.

Biography:

Denise Cekaunaskas Kalil Lauand received the Bachelor´s degree in Physical Therapy from the Universidade Paulista - UNIP (2004). Upon graduation, she acted in the field of orthopedic physical therapy as well as in the prevention and rehabilitation of musculoskeletal disorders. She is currently a Master´s student at the Universidade Nove de Julho´s (UNINOVE) Rehabilitation Sciences program, working under the supervision of Prof. Dr. Raquel Agnelli Mesquita Ferrari.

Abstract:

Presenter Local Time: 9:50 AM-10:00 AM
Chronic neck pain is a persistent condition affecting the spinal region, resulting in pain and restricted mobility. The management of neck pain often involves manual therapies, encompassing both passive and active interventions, aimed at alleviating pain, enhancing function, improving mobility, motor control, and reducing inflammatory processes. This pain can persist for at least three months and is considered non-specific when it is not associated with any specific underlying condition, such as inflammatory rheumatic disease, osteoporosis, cancer, or radiculopathy. The use of lasers and LEDs for photobiomodulation (PBM) represents an advantageous approach to treating neck pain, given their demonstrated therapeutic efficacy in the literature. Moreover, these resources are non-invasive and easy to apply, making them an attractive option for both patients and healthcare professionals. Therefore, the aim of this study is to evaluate the effects of Pompage associated or not with PBM, using a cluster of LEDs, on pain and neck disability. This controlled, randomized, and blinded clinical study includes participants of both genders, aged 18 to 45, with non-specific chronic neck pain will be included. Participants will be randomized into two groups: (1) Pompage (n=28) focusing only on manual therapy through Pompage technique and (2) Pompage + PBM Group (n=28) involving the same procedures as the first group, followed by PBM with a LED cluster applied for 10 minutes to the neck region. The treatment protocol consists of 10 sessions, three times per week, excluding weekends. For PBM, a cluster comprising 264 LEDs (8 mW; 4.89J; 9.6 J/cm²; 16 mW/cm² per LED) will be used, with 132 red (660nm) and 132 infrared (850nm) LEDs. Pain and functional disability will be assessed using the visual analog scale (VAS) and Neck Pain Disability Index before and after the intervention. The resulting data will be submitted to statistical analysis considering α=0.05.

Biography:

Drielli Viana Souza, graduated in Dentistry from Faculdade Nove de Julho, Specialized in Oral and Maxillofacial Surgery and Traumatology from Faculdade IBECO and Master's Degree in Biophotonics in Health Sciences from Universidade Nove de Julho.

Abstract:

Presenter Local Time:8:25 AM-8:35 AM
Osteomolecular surgeries are common worldwide, causing a socioeconomic impact on the country. The use of external agents such as composite biomaterials is crucial to expedite bone repair and control potential infections, thus advancing patient rehabilitation. The study of photobiomodulation therapy associated with the use of non-scarce carbon biomaterials is significant. OBJECTIVE: To evaluate the effect of Photobiomodulation Therapy associated with carbon material impregnated with silver nanoparticles on bacterial control, cell growth, and the bone repair process in an experimental model of bone injury in rat tibia. MATERIALS AND METHODS: Wistar rats were randomly distributed into groups: G1 (healthy rats); BF (bone defect); BF NT (BF without treatment); BF+C (BF with carbon material); BF+CNP (BF with carbon material associated with silver nanoparticles) BF+CNP+PBM (BF with carbon material associated with silver nanoparticles and photobiomodulation); Local PBM (808nm photobiomodulation, 100mW, 6J, 60s). The animals will be euthanized at 30-60-90 days after the injury. Blood and tibia samples will be collected for biochemical (alkaline and acid phosphatase) and morphological (histological) analyses. Functional study will include biomechanical tests (shear, resistance, and flexibility).

Biography:

I am currently a Master's degree student in Biophotonics applied to Health Sciences at UNINOVE, supported by a CAPES scholarship. I earned my undergraduate degree in Biomedicine from the Universidade Mogi das Cruzes in 1992. In 2004, I completed a specialization in Hospital Administration and Health Systems at the Business Institute, Faculdade Getúlio Vargas. Additionally, I have experience in laboratory and clinical research acquired through more than 20 years of work at the Federal University of São Paulo.

Abstract:

Presenter Local Time: 8:55 AM-9:05 AM
Macrophages polarized to the M1 profile play a crucial role in the inflammatory process, being essential in the body's immune response to pathogens, tissue injuries, and other forms of cellular stress, modulating, through their products, the different phases of cellular repair. Many anti-inflammatory drugs have been used for the treatment of inflammatory processes, with one of the most common in clinical practice being the disodium dexamethasone phosphate (FD-Dexa). Despite being very effective, FD-Dexa can cause numerous side effects. The risk of adverse effects, some of which may be potentially severe, increases with higher doses and longer treatment durations. In the literature, photobiomodulation (PBM) has demonstrated its ability to attenuate the inflammatory process through various mechanisms, including the modulation of macrophage activity and secretory products, inducing polarization to an anti-inflammatory phenotype (M2), which is associated with inflammation control and tissue repair promotion. Thus, both isolated PBM and its combination with corticosteroids show potential effects on the modulation of inflammatory cytokine expression by M1 macrophages. The objective of this study is to evaluate the effects of PBM, with or without the addition of disodium dexamethasone phosphate (FD-Dexa), on the secretion of inflammatory cytokines and nitric oxide synthesis in M1-polarized macrophages. For this study, J774 macrophages will be used and evaluated in the following groups: (1) Control Group - J774 cells without any treatment; (2) M1 Group - J774 activated to the M1 phenotype; (3) M1 + PBM Group J774 activated to the M1 phenotype and submitted to PBM; (4) M1 + FD-Dexa 2µM Group including the Dexa treatment; (5) M1 + FD-Dexa 4µM Group; (6) M1 + PBM + FD-Dexa 2µM Group and (7) M1 + PBM + FD-Dexa 4µM Group. Duplicate analyses will be conducted for all experimental groups. The J774 macrophages will be activated to the M1 phenotype using Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution, LPS 1µg/mL and 0.2µg/mL interferon-gamma (IFN-?). They will be incubated for 2 hours at 37°C in a humidified atmosphere with 5% CO2. The PBM groups will be irradiated using an Aluminum Gallium Arsenide laser (780 nm, 70 mW, 17.5J/cm2, 1J) and FD-Dexa 2µM and 4µM will be added to the corresponding groups, and then the cells of all experimental groups will be incubated at 37°C, 5% CO2 for 24 and 48 hours. At the end of the incubation period, culture medium will be collected, total protein quantified using a Nano spectrophotometer (Nanodrop), and then analysis of IL-6, IL-1β, and TNF-α synthesis will be performed using ELISA technique and the nitric oxide (NO) synthesis using the Griess method. Experiments will be conducted in triplicate, and data will be subjected to statistical analysis.

Biography:

Silmara Brasileiro Quaresma,obtained her bachelor's degree in pharmacy and biochemistry, and post-graduated in pharmaceutical care and clinicalpharmacy from Nove de Julho University.Specialization in aesthetic pharmacy from the Institute ofResearch and Education in Health of São Paulo.

Abstract:

Presenter Local Time: 8:05 AM-8:15 AM
According to the United Nations (UN) , the number of elderly people has tripled, from around 260 million in 1980 to 761 million in 2021. Aging is an inevitable process, but it is essential to understand its implications for health. Factors such as sunexposure, smoking and alcohol accelerate skin aging, damaging cells and requiring preventive care for a healthier life. Studies indicate the efficacy and safety of the simultaneous approach of Hyaluronic Acid (HA) fillers combined with Laser or LED therapies in rejuvenation.
Objective: To evaluate the effect of the combination ofphotobiomodulation therapy and hyaluronic acid in an in vitro model of photoaging using UVlight and cigarette smoke extract (CSE) on keratinocytes and fibroblasts. Keratinocytes andfibroblasts will be incubated at 37°C and 5% CO2 until reaching subconfluence, where they will be irradiated with UV light for 24 hours. After this period, cigarette smoke extract(2.5%) can be added or not and irradiated or not with Laser (diode, 660 nm, 30 mW for 180s) and/or HA (0.1 mg/ml ). After adequate culture time, the cells will be diluted and placed in24-well plates, and the assay will be performed in triplicate. Cell viability and proliferation(MTT), quantification of IL-1,TNF, IL-6, IL-10, IL-8, TGF (ELISA), determination ofglutathione peroxidase (GPx), Superoxide activities will be carried out dismutase (SOD) andcatalase, as well as collagen quantification. Data will be analyzed and graphs created using GraphPad Prism 5.0 software. Data will be submitted to one-way ANOVA followed by Newman-Keuls post-test for group comparisons.

Biography:

Abstract:

Presenter Local Time: 8:35-8:45
Canine otitis externa is a common dermatological inflammatory disease of the external ear canal and ear pina. Etiology is multifactorial and involves primary, predisposing and/or perpetuating factors. Clinical signs include pain, pruritus, alopecia, erythema, head shaking and malodour. Treatment typicallly involves the use of topical otic antibiotics, anti-inflammatories or analgesics alone or in combination. However, some topical solutions may cause ototoxicity, adverse reactions and/or increase the odds for multidrug-resistant bacteria. Photobiomodulation (PBM), also known as LLLT (low-level laser therapy) is a non-invasive method that contributes to pain relief and reduction of inflammation contributing to tissue healing. Thus, PBM may be an alternative to conventional drug treatment. The aim of this study is to compare the potential benefits of PBM in the treatment of canine otitis externa to conventional treatment with topical otic drugs. Dogs will be randomically allocated in 3 different groups: group 1 (control), treated only with a commerical cleaning otic solution; group 2 (cleaning solution + conventional drug treatment); and group 3 (cleaning solutions + PBM therapy). Our hypothesis is that PBM anti-inflammatory effect can completely heal otitis without needing of antibiotics, antifungals and/or anti-inflammatories. A p<0.05 will be the level of statistical significance.

Biography:

Abstract:

Presenter Local Time: 10:00 AM-10:10 AM
The importance of thyroid hormones on the skin has been known for a long time. Still, the mechanisms of thyroid hormone regulation in skin cells, especially at different concentrations of melanin, have been little studied. This study aimed to analyze online databases of gene expression associated with thyroid hormone metabolism in the skin.

Methods: We analyzed the datasets in the Gene Expression Omnibus (GEO) repository by searching with the keyword skin cells and filtering for tissue and homo sapiens studies. We evaluated the following genes: SLC16A2, SLC16A10, SLC10A1, SLC7A5, SLC7A8, DIO1, DIO2, DIO3, THRA and THRB. The data was log-transformed before the analyses, and the t-test or ANOVA was used. We analyzed the data using the IBM SPSS Statistics for Windows, Version 29.0. Results: We identified 23 datasets. Nine were excluded (2 neoplastic, one repeated, one blood cell, one hair follicle and four compared multiple tissues). A total of 330 samples were considered in (GSE60317, GSE58573, GSE45512, GSE53552, GSE51028, GSE50790, GSE52471, GSE41905, GSE36287, GSE30768, GSE27165, GSE32924, GSE32473 and GSE27887). Three transcripts (SLC10A1, DIO1 and THRB) information was unavailable in GSE52471. Three studies used sequencing technology, and the rest used an array. All transcripts were detected in all tissues, even considering the disease-associated samples. The UVB exposition did not modify the expression of the studied transcripts. Conclusion: Thyroid metabolism-related genes were expressed in skin cells and may vary in inflammatory disease, but UVB seems to have little impact on those transcripts' expression.